Yabe T, Kast A
Department of Experimental Pathology, Nippon Boehringer Ingelheim Co., Ltd., Hyogo, Japan.
J Toxicol Sci. 1988 Nov;13(4):179-91. doi: 10.2131/jts.13.179.
The optimum pH of serum alkaline phosphatase (alk. P., EC 3.1.3.1) was investigated in adult male rats, mice and rabbits after fasting or after the induction of cholestasis by a single oral dose of 200 mg/kg alpha-naphthylisothiocyanate (ANIT). The nature of serum alk.P. was also studied with the inhibitory effects of three L-amino acids (L-phenylalanine, L-homoarginine and L-cystine) and by electrophoresis. In the rat, fasting as well as ANIT-treatment shifted the optimum pH of alk.P. from 9.4 to 10.0 due to a decrease in intestinal isoenzymes or due to newly appeared liver isoenzymes, while no shifting occurred in mouse and rabbit.
在成年雄性大鼠、小鼠和兔子中，研究了禁食或单次口服200mg/kgα-萘基异硫氰酸酯（ANIT）诱导胆汁淤积后血清碱性磷酸酶（alk.P.，EC 3.1.3.1）的最佳pH值。还通过三种L-氨基酸（L-苯丙氨酸、L-高精氨酸和L-胱氨酸）的抑制作用以及电泳研究了血清alk.P.的性质。在大鼠中，禁食以及ANIT处理使alk.P.的最佳pH值从9.4变为10.0，这是由于肠道同工酶减少或新出现的肝脏同工酶所致，而在小鼠和兔子中未发生变化。
