微芯片设备上的毛细管电泳能否对尿苷二磷酸葡萄糖醛酸转移酶1A1 TATA盒多态性进行基因分型？

Is capillary electrophoresis on microchip devices able to genotype uridine diphosphate glucuronosyltransferase 1A1 TATA-box polymorphisms?

作者信息

Minucci Angelo, Canu Giulia, De Bonis Maria, Delibato Elisabetta, Capoluongo Ettore

机构信息

Laboratory of Clinical Molecular Diagnostics and Personalized Medicine, Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy.

出版信息

J Sep Sci. 2014 Jun;37(12):1521-3. doi: 10.1002/jssc.201400235. Epub 2014 May 2.

DOI:10.1002/jssc.201400235

PMID:24687976

Abstract

In this commentary, we focused our attention on capillary electrophoresis. It achieves the efficient separation of molecular species by the application of high voltages to samples in solution. Actually, capillary electrophoresis can be performed on microchip devices, based on an automated and miniaturized electrophoresis system, based on lab-on-a-chip technology. By this technology it is possible to separate nucleic acid fragments (DNA or RNA) with respect to sizing accuracy and sizing resolution. Currently, two automated capillary electrophoresis on microchips devices are available: the Agilent 2100 Bioanalyzer and the Experion™ Automated Electrophoresis System. In this study, we evaluated if the CE is able to distinguish the three uridine diphosphate glucuronosyltransferase 1A1 TATA-box genotypes.

摘要

在本评论中，我们将注意力集中在毛细管电泳上。它通过对溶液中的样品施加高电压来实现分子物种的高效分离。实际上，基于芯片实验室技术的自动化和小型化电泳系统，毛细管电泳可以在微芯片设备上进行。通过这项技术，可以在大小准确性和大小分辨率方面分离核酸片段（DNA或RNA）。目前，有两种微芯片设备上的自动化毛细管电泳可供使用：安捷伦2100生物分析仪和Experion™自动化电泳系统。在本研究中，我们评估了毛细管电泳是否能够区分三种尿苷二磷酸葡萄糖醛酸基转移酶1A1 TATA盒基因型。