Abranches Andrea D, Soares Fernanda V M, Junior Saint-Clair G, Moreira Maria Elisabeth L
Instituto Nacional de Saúde da Mulher, Criança e Adolescente Fernandes Figueira (Fiocruz), Rio de Janeiro, RJ, Brazil.
Instituto Nacional de Saúde da Mulher, Criança e Adolescente Fernandes Figueira (Fiocruz), Rio de Janeiro, RJ, Brazil.
J Pediatr (Rio J). 2014 Jul-Aug;90(4):384-8. doi: 10.1016/j.jped.2013.11.001. Epub 2014 Mar 29.
to analyze the changes in human milk macronutrients: fat, protein, and lactose in natural human milk (raw), frozen and thawed, after administration simulation by gavage and continuous infusion.
an experimental study was performed with 34 human milk samples. The infrared spectrophotometry using the infrared analysis equipment MilkoScan Minor® (Foss, Denmark) equipment was used to analyze the macronutrients in human milk during the study phases. The analyses were performed in natural (raw) samples and after freezing and fast thawing following two steps: gavage and continuous infusion. The non-parametric Wilcoxon test for paired samples was used for the statistical analysis.
the fat content was significantly reduced after administration by continuous infusion (p<0.001) during administration of both raw and thawed samples. No changes in protein and lactose content were observed between the two forms of infusion. However, the thawing process significantly increased the levels of lactose and milk protein.
the route of administration by continuous infusion showed the greatest influence on fat loss among all the processes required for human milk administration.
分析天然母乳（生鲜）、冷冻和解冻后的母乳在经灌胃模拟给药和持续输注后的宏量营养素（脂肪、蛋白质和乳糖）变化。
对34份母乳样本进行了一项实验研究。在研究阶段，使用MilkoScan Minor®红外分析设备（丹麦福斯公司）通过红外分光光度法分析母乳中的宏量营养素。分析在天然（生鲜）样本以及冷冻和快速解冻后分两步进行：灌胃和持续输注。配对样本的非参数威尔科克森检验用于统计分析。
在生鲜样本和解冻样本给药期间，持续输注给药后脂肪含量显著降低（p<0.001）。两种输注方式之间未观察到蛋白质和乳糖含量的变化。然而，解冻过程显著提高了乳糖和乳蛋白水平。
在母乳给药所需的所有过程中，持续输注给药途径对脂肪损失的影响最大。
