Lin H C, Piing J T, Tseng Y M, Chao C F
Department of Surgery, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Proc Natl Sci Counc Repub China B. 1988 Oct;12(4):215-21.
Epidermal growth factor is an important element in maintaining keratinocyte proliferation and maturation. To evaluate its effect on keratinocyte growth in vitro, human foreskins were cultured. The epidermal keratinocyte growth in culture was separated into two stages by a conditional medium: the proliferation stage, in which the cells were maintained in a monolayer; and the differentiation stage, in which the cells grew to stratification and keratinization. The keratinocytes were cultured in various concentrations of epidermal growth factor, and their morphology and growth behavior were closely observed. Our results demonstrated that cultured keratinocytes grew in a confluent layer under the influence of epidermal growth factor. In contrast, in a culture without epidermal growth factor, the proliferation rate of cultured keratinocytes slowed down and eventually the cells stopped growing. During serum stimulation, with or without additional exogenous epidermal growth factor, the cultured keratinocytes grew continuously to the normal terminal differentiation. Under this two-stage culture model, the cultured keratinocytes could grow into an intact sheet of graftable epidermis.
表皮生长因子是维持角质形成细胞增殖和成熟的重要因素。为了评估其对体外角质形成细胞生长的影响，培养了人包皮。培养物中的表皮角质形成细胞生长通过条件培养基分为两个阶段：增殖阶段，细胞维持在单层状态；分化阶段，细胞生长至分层和角质化。将角质形成细胞培养在不同浓度的表皮生长因子中，并密切观察其形态和生长行为。我们的结果表明，培养的角质形成细胞在表皮生长因子的影响下呈汇合层生长。相反，在没有表皮生长因子的培养物中，培养的角质形成细胞的增殖速率减慢，最终细胞停止生长。在血清刺激期间，无论有无额外的外源性表皮生长因子，培养的角质形成细胞都持续生长至正常的终末分化。在这种两阶段培养模型下，培养的角质形成细胞可以生长成完整的可移植表皮片。
