Xu Li, Wang Sheng, Lv Yingnian, Son Young-A, Cao Derong
School of Chemistry and Chemical Engineering, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510641, China; College of Chemistry Science & Technology, Zhanjiang Normal University, Zhanjiang 524048, China.
School of Chemistry and Chemical Engineering, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510641, China; College of Chemistry Science & Technology, Zhanjiang Normal University, Zhanjiang 524048, China; State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jul 15;128:567-74. doi: 10.1016/j.saa.2014.03.001. Epub 2014 Mar 14.
A new photochromic diarylethene derivative bearing rhodamine 6G dimmer as a fluorescent molecular probe is designed and synthesized successfully. All the compounds are characterized by nuclear magnetic resonance and mass spectrometry. The bisthienylethene-rhodamine 6G dyad exhibit excellent phtochromism with reversibly color and fluorescence changes alternating irradiation with ultraviolet and visible light. Upon addition of Hg(2+), its color changes from colorless to red and its fluorescence is remarkably enhanced. Whereas other ions including K(+), Na(+), Ca(2+), Mg(2+), Fe(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Mn(2+), Pb(2+), Ni(2+), Fe(3+), Al(3+), Cr(3+) and so on induce basically no spectral changes, which constitute a highly selective and sensitive photoswitchable fluorescent probe toward Hg(2+). Furthermore, by means of laser confocal scanning microscopy experiments, it is demonstrated that this probe can be applied for live cell imaging and monitoring Hg(2+) in living lung cancer cells with satisfying results, which shows its value of potential application in environmental and biological systems.
成功设计并合成了一种新型的带有罗丹明6G二聚体作为荧光分子探针的光致变色二芳基乙烯衍生物。所有化合物均通过核磁共振和质谱进行表征。双噻吩乙烯-罗丹明6G二元化合物表现出优异的光致变色性能，在紫外光和可见光交替照射下颜色和荧光发生可逆变化。加入Hg(2+)后，其颜色从无色变为红色，荧光显著增强。而包括K(+)、Na(+)、Ca(2+)、Mg(2+)、Fe(2+)、Co(2+)、Ni(2+)、Cu(2+)、Zn(2+)、Mn(2+)、Pb(2+)、Ni(2+)、Fe(3+)、Al(3+)、Cr(3+)等在内的其他离子基本不引起光谱变化，这构成了一种对Hg(2+)具有高选择性和高灵敏度的光开关荧光探针。此外，通过激光共聚焦扫描显微镜实验表明，该探针可用于活细胞成像，并能对肺癌活细胞中的Hg(2+)进行监测，结果令人满意，显示出其在环境和生物系统中的潜在应用价值。
