Martin J R, Suzuki S
National Institutes of Health, Laboratory of Experimental Neuropathology, Bethesda, MD 20892.
Acta Neuropathol. 1989;77(4):402-11. doi: 10.1007/BF00687375.
In mice surviving intracerebral inoculation with herpes simplex virus type 2 (HSV-2), recurrent infection was induced using high-dose immunosuppressive treatment. Targets of reactivated infection were identified in serial sections of heads using immunoperoxidase methods to detect viral antigen. Peripheral targets were cranial sensory and autonomic ganglia, peripheral nerves, and many non-neural structures. Central neural tissues included retina, optic nerve and tract, lateral geniculate nucleus and superior colliculus. Brain stem nuclei containing antigen were chiefly those associated with the trigeminal, facial, glossopharyngeal or vagus nerves. A few white matter regions were also positive. This is the first study using antigen methods to show that the central nervous system can be an important target of recurrent HSV infection in an animal model. Patterns of antigen-containing structures suggest that neural connections are important determinants of sites of reactivated infection. Decalcification of bone permits study of antigen distribution in tissues retaining their anatomical relationships.
在用2型单纯疱疹病毒（HSV-2）进行脑内接种后存活的小鼠中，使用高剂量免疫抑制治疗诱导复发性感染。采用免疫过氧化物酶方法检测病毒抗原，在头部连续切片中确定再激活感染的靶点。外周靶点包括颅感觉和自主神经节、外周神经以及许多非神经结构。中枢神经组织包括视网膜、视神经和视束、外侧膝状体和上丘。含有抗原的脑干核主要是与三叉神经、面神经、舌咽神经或迷走神经相关的核。少数白质区域也呈阳性。这是第一项使用抗原方法表明在动物模型中中枢神经系统可能是复发性HSV感染重要靶点的研究。含抗原结构的模式表明神经连接是再激活感染部位的重要决定因素。骨脱钙有助于研究保留其解剖关系的组织中的抗原分布。
