Rasheed K, Atta H, Taha Tf, Azmy O, Sabry D, Selim M, El-Sawaf A, Bibars M, Ramzy A, El-Garf W, Anwar M
Obstetrics and Gynecology Department, Faculty of Medicine, Cairo University.
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University.
J Stem Cells Regen Med. 2010 Oct 30;6(3):157-64. doi: 10.46582/jsrm.0603009. eCollection 2010.
To confirm the hypothesis of the presence of a possible endometriosis inducing factor(s) (EIF) in the blood of women with endometriosis.
Forty infertile women were studied. The study group compromised of fifteen women of each three different degrees of endometriosis and fifteen women without endometriosis as a control group. Stem cells are characterized by being spindle shaped and proliferate in appropriate culture indefinitely. The women sera were co-cultured with mesenchymal stem cells (MSCs) which were followed up weekly to look for morphological changes and to detect Annexin 1 marker and ß-actin gene by reverse transcriptase polymerase chain reaction.
MSCs cultured with sera of cases with, mild, moderate and severe endometriosis, showed morphological changes to be columnar and cuboidal shaped cells -resembling endometrial cells and glands- by the 4th week in 60%, 60% & 100% respectively. These cells were detected from as early as the first week in women with moderate and severe types (20% for each group). The percentage of the change into endometrial like cells increased among the three groups where it was 30±25.8%, 45±29.9% and 75±37.9% respectively. Moreover, increasing number of endometrial like cells are detected weekly, the more severe the disease is. None of the cultures of serum of the control group had made such changes all over the study. Furthermore, with more differentiation there was a considerable decrease in number of stem cells. These differentiated cells expressed the Annexin-1 marker.
It was evident that serum of women with endometriosis posses a factor(s) that enables the MSCs to be transformed into endometrial like cells and glands in vitro. This finding supports a new theory for the etiology of endometriosis. This observation may have a tremendous effect on the therapeutic implications of this debilitating condition.
证实子宫内膜异位症患者血液中存在可能的子宫内膜异位症诱导因子（EIF）这一假说。
对40名不孕女性进行研究。研究组由患有三种不同程度子宫内膜异位症的各15名女性以及15名无子宫内膜异位症的女性作为对照组组成。干细胞的特征是呈纺锤形且在合适的培养条件下可无限增殖。将女性血清与间充质干细胞（MSC）共培养，每周进行随访以观察形态变化，并通过逆转录聚合酶链反应检测膜联蛋白1标志物和β-肌动蛋白基因。
与轻度、中度和重度子宫内膜异位症患者血清共培养的MSC，在第4周时分别有60%、60%和100%呈现出形态变化，变为柱状和立方状细胞，类似于子宫内膜细胞和腺体。在中度和重度类型的女性中，最早在第1周就检测到了这些细胞（每组20%）。三组中转变为子宫内膜样细胞的百分比有所增加，分别为30±25.8%、45±29.9%和75±37.9%。此外，每周检测到的子宫内膜样细胞数量不断增加，疾病越严重增加得越多。在整个研究过程中，对照组血清培养物均未出现此类变化。此外，随着分化程度的增加，干细胞数量显著减少。这些分化细胞表达膜联蛋白-1标志物。
显然，子宫内膜异位症患者的血清中存在一种因子，能够使MSC在体外转化为子宫内膜样细胞和腺体。这一发现支持了子宫内膜异位症病因学的新理论。这一观察结果可能对这种使人衰弱的疾病的治疗产生巨大影响。
