Parand Alireza, Zolghadri Jale, Nezam Mozhgan, Afrasiabi Abdolreza, Haghpanah Sezaneh, Karimi Mehran
Iranian Hospital, Dubai, UAE.
Infertility Research Center, Gynecology and Obstetrics Department, Shiraz University of Medical Sciences, Shiraz, IR Iran.
Iran Red Crescent Med J. 2013 Dec;15(12):e13708. doi: 10.5812/ircmj.13708. Epub 2013 Dec 5.
Recurrent pregnancy loss (RPL) is a common health problem. The polymorphisms G20210A of prothrombin gene (FII G 20210A), and G 1691A of factor V gene (Factor V Leiden, FVL) are the most extensively studied thrombophilic mutations in association to recurrent miscarriage.
To determine the frequency of FII G20210A and FVL polymorphisms as well as protein C and protein S deficiency in a series of patients with RPL compared with control group.
The study group included 90 randomly selected patients with three or more consecutive miscarriages with the same partner in <20 weeks gestation in 2012. The control population consisted of 44 age-matched women with at least one live born children and no history of pregnancy loss. Functional activity of protein C and S, activated protein C resistance, FVL assay by polymerase chain reaction and prothrombin gene mutation were assessed. The polymorphism frequencies were recorded for each group and comparisons were made.
The mean functional activity of protein C and protein S were not significantly different between case and control groups (P >0.05). Frequency of protein C deficiency was also not significantly different between the case and control groups (P=0.906), but frequency of protein S deficiency was significantly higher in patients than controls (P=0.03). Genotype pattern of the patients and healthy individuals were not significantly different with regard to either FVL or Prothrombin G20210A (P > 0.05).
We determined a significant higher frequency of protein S deficiency in patients with RPL compared with controls. But the frequency of protein C deficiency and the frequency of two common thrombophilic mutations (Factor V Leiden and Prothrombin G20210A), were not significantly different between patients with recurrent miscarriage and healthy women.
复发性流产（RPL）是一个常见的健康问题。凝血酶原基因多态性G20210A（FII G 20210A）和因子V基因多态性G 1691A（因子V莱顿突变，FVL）是与复发性流产相关的研究最为广泛的血栓形成倾向突变。
确定复发性流产患者中FII G20210A和FVL多态性以及蛋白C和蛋白S缺乏症的发生率，并与对照组进行比较。
研究组包括2012年随机选取的90例妊娠<20周、与同一伴侣连续发生三次或三次以上流产的患者。对照组由44名年龄匹配的女性组成，她们至少生育过一个存活婴儿且无流产史。评估了蛋白C和蛋白S的功能活性、活化蛋白C抵抗、通过聚合酶链反应检测FVL以及凝血酶原基因突变。记录每组的多态性频率并进行比较。
病例组和对照组之间蛋白C和蛋白S的平均功能活性无显著差异（P>0.05）。病例组和对照组之间蛋白C缺乏症的发生率也无显著差异（P = 0.906），但患者中蛋白S缺乏症的发生率显著高于对照组（P = 0.03）。患者和健康个体在FVL或凝血酶原G20210A方面的基因型模式无显著差异（P>0.05）。
我们发现复发性流产患者中蛋白S缺乏症的发生率显著高于对照组。但复发性流产患者与健康女性之间蛋白C缺乏症的发生率以及两种常见的血栓形成倾向突变（因子V莱顿突变和凝血酶原G20210A）的发生率无显著差异。
