Maxisorp 放射变应原吸附试验。一种检测培养液中抗原特异性人 IgE 的灵敏方法。

Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids.

作者信息

Poulsen L K, Pedersen M F, Malling H J, Søndergaard I, Weeke B

机构信息

Laboratory of Medical Allergology, Medical Dept. TTA 7542, State University Hospital, Copenhagen, Denmark.

出版信息

Allergy. 1989 Apr;44(3):173-80. doi: 10.1111/j.1398-9995.1989.tb02258.x.

DOI:10.1111/j.1398-9995.1989.tb02258.x

PMID:2469345

Abstract

For determination of allergen-specific IgE in cell culture supernatants and other highly diluted IgE preparations a radioallergosorbent test (RAST) based on high adsorption polystyrene test tubes has been developed ("Maxisorp RAST"). Cladosporium herbarum extract was used as a model allergen but timothy grass pollen, house dust mite and dog dander showed similar results. The test showed specificities of both allergen and immunoglobulin isotype and significant correlations (r = 0.67-0.88) with established RAST procedures were found. Based on immunosorbent-purified allergen-specific IgE the estimated sensitivity was within the order of 150-300 pg allergen-specific IgE per ml. The within-assay variation was 4-9% and the inter-assay-variation 17-29%. The Maxisorp RAST is useful as an inhibition assay for quantitating allergenic activity down to 0.1 biological units/ml of allergen extracts.

摘要

为了测定细胞培养上清液和其他高度稀释的IgE制剂中的变应原特异性IgE，已开发出一种基于高吸附聚苯乙烯试管的放射变应原吸附试验（RAST）（“Maxisorp RAST”）。用草本枝孢菌提取物作为模型变应原，但梯牧草花粉、屋尘螨和狗毛屑显示出相似的结果。该试验显示了变应原和免疫球蛋白同种型的特异性，并且发现与既定的RAST程序具有显著相关性（r = 0.67 - 0.88）。基于免疫吸附纯化的变应原特异性IgE，估计灵敏度在每毫升150 - 300 pg变应原特异性IgE的范围内。批内变异为4 - 9%，批间变异为17 - 29%。Maxisorp RAST可用作抑制试验，用于定量低至0.1生物单位/毫升变应原提取物的变应原活性。