Poor M L, Santa P F, Sittampalam G S
Lilly Research Laboratories, Eli Lilly & Company, Indianapolis, Indiana 46285.
Anal Biochem. 1988 Nov 15;175(1):191-5. doi: 10.1016/0003-2697(88)90377-6.
A method has been developed which allows the simultaneous immunodetection of more than one type of protein on the same nitrocellulose membrane. This procedure does not require special labeling of samples or elution of antibodies from the membrane as do the alternatives cited in the literature (1,2). Proteins are separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to the membrane before specific immunostaining with either peroxidase/4-chloro-1-naphthol or immunogold/silver staining. Antigen identity is visually determined by the formation of different-colored precipitates on the membrane. This innovation in protein blotting offers a savings in time and reagents as well as permitting identification of closely spaced bands with certainty.
已开发出一种方法，可在同一硝酸纤维素膜上同时免疫检测多种类型的蛋白质。与文献（1,2）中引用的其他方法不同，该程序不需要对样品进行特殊标记或从膜上洗脱抗体。蛋白质通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离，然后在进行过氧化物酶/4-氯-1-萘酚或免疫金/银染色的特异性免疫染色之前，通过电泳转移到膜上。通过膜上形成不同颜色的沉淀来直观地确定抗原身份。蛋白质印迹法的这一创新节省了时间和试剂，同时也能确定地识别间隔很近的条带。
