Nakajima T, Ara W, Saito K, Moores J E, Millar B C, Matsuda M
Br J Biomed Sci. 2014;71(1):19-28. doi: 10.1080/09674845.2014.11669958.
Southern hybridisation shows that urease-negative (UN) Campylobacter lari JCM2530(T) carries two putative major outer membrane protein (MOMP) genes. Sequences of approximately 2.1 kbp, encoding non-coding (NC) regions, with possible open reading frames (ORFs) for MOMP (porA1 or porA2) of approximately 1.2 kbp, NC regions and partial and putative Cla_0435 or Cla_1109 ORFs were identified in all five UN C. lari isolates examined, following polymerase chain reaction (PCR) cloning and sequencing. Each putative MOMP structural gene carried start and stop codons and ribosome binding sites of 1236-1278 bp in length. The putative sigma70 transcriptional promoter and the hypothetical rho-independent transcription terminator structures were also seen. Using Northern hybridisation, there was in vivo monocistronic MOMP gene transcription. In addition, in a Japanese urease-positive thermophilic Campylobacter (UPTC) CF89-12 strain, the porA1 gene locus, including an extra gene (approximately 2000 bp in length) was identified. The extra gene may occur within the porA1 gene locus in the eight UPTC isolates of the 23 C. lari isolates examined. Thus, a genetic heterogeneity occurred within the porA1 gene locus from some of the C. lari organisms including the UPTC CF89-12.
Southern杂交显示，脲酶阴性（UN）的空肠弯曲菌JCM2530(T)携带两个假定的主要外膜蛋白（MOMP）基因。在所有检测的5株UN空肠弯曲菌分离株中，经聚合酶链反应（PCR）克隆和测序后，鉴定出了约2.1kbp的序列，其编码非编码（NC）区域，带有约1.2kbp的MOMP（porA1或porA2）的可能开放阅读框（ORF）、NC区域以及部分和假定的Cla_0435或Cla_1109 ORF。每个假定的MOMP结构基因都带有起始和终止密码子以及长度为1236 - 1278bp的核糖体结合位点。还发现了假定的sigma70转录启动子和假定的不依赖rho的转录终止子结构。通过Northern杂交，发现存在体内单顺反子MOMP基因转录。此外，在一株日本脲酶阳性嗜热弯曲菌（UPTC）CF89 - 12菌株中，鉴定出了porA1基因位点，包括一个额外基因（长度约2000bp）。在所检测的23株空肠弯曲菌分离株中的8株UPTC分离株中，额外基因可能出现在porA1基因位点内。因此，在包括UPTC CF89 - 12在内的一些空肠弯曲菌生物体的porA1基因位点内发生了遗传异质性。
