Norrby E, Chiodi F, Whalley A, Parks E, Nauclér A, Costa C M, Torstensson R, Biberfeld G
Department of Virology, Karolinska Institutet, School of Medicine, Stockholm, Sweden.
AIDS. 1989 Jan;3(1):17-20.
A 23-amino-acid-long peptide (AIEKYLEDQAQLNAWGCAFRQVC) representing the transmembranous protein gp32 in SIVmac was used in site-directed enzyme-linked immunosorbent assay (ELISA) for detection of HIV-2-specific antibodies in 567 sera from Bissau, Guinea Bissau. Ninety out of the 567 sera were identified to contain HIV-2 antibodies by whole antigen ELISA and Western blot assays. The peptide ELISA correctly identified 89 out of these 90 seropositives (sensitivity 98.9%). Three sera falsely interpreted to be positive were encountered (specificity 99.4%). The HIV-2 peptide was also used for testing of 93 HIV-1-positive Swedish sera. None of these sera reacted. Site-directed serology employing synthetic peptides should be considered for application as a screening assay.
一个代表猴免疫缺陷病毒（SIVmac）跨膜蛋白gp32的23个氨基酸长的肽段（AIEKYLEDQAQLNAWGCAFRQVC）被用于位点特异性酶联免疫吸附测定（ELISA），以检测来自几内亚比绍比绍的567份血清中的HIV-2特异性抗体。通过全抗原ELISA和蛋白质印迹分析，在567份血清中有90份被鉴定含有HIV-2抗体。肽段ELISA正确鉴定出这90份血清阳性样本中的89份（敏感性为98.9%）。遇到3份被错误解释为阳性的血清（特异性为99.4%）。该HIV-2肽段也用于检测93份HIV-1阳性的瑞典血清。这些血清均无反应。采用合成肽的位点特异性血清学检测应被考虑用作筛查试验。
