与釉质蛋白存在免疫相关性的人和小鼠牙骨质蛋白。

Human and mouse cementum proteins immunologically related to enamel proteins.

作者信息

Slavkin H C, Bessem C, Fincham A G, Bringas P, Santos V, Snead M L, Zeichner-David M

机构信息

Department of Basic Sciences (Biochemistry), School of Dentistry, University of Southern California, Los Angeles 90089-0191.

出版信息

Biochim Biophys Acta. 1989 Apr 25;991(1):12-8. doi: 10.1016/0304-4165(89)90021-4.

DOI:10.1016/0304-4165(89)90021-4

PMID:2469482

Abstract

SDS-polyacrylamide gel electrophoresis, immunoblot and amino acid composition analyses were applied to human and mouse acellular cementum proteins immunologically related to enamelins and amelogenins. In this analysis, anti-mouse amelogenin, anti-human enamelin and synthetic peptide (e.g., -LPPHPGHPGYIC-) antibodies were shown to cross-react with tooth crown-derived enamelin with a molecular mass of 72,000 Da (72 kDa), amelogenins (26 kDa), and also to four human cementum proteins (72, 58, 50 and 26 kDa) and two mouse cementum proteins (72 and 26 kDa). Each of the antibodies recognized tooth root-derived cementum polypeptides which share one or more epitopes with tooth crown-derived enamel proteins. The molecular mass and isoelectric points for crown-derived and root-derived enamel-related proteins were similar. Analysis of human and mouse cementum proteins revealed a characteristic amino acid composition enriched in glutamyl, serine, glycine, alanine, proline, valine and leucine residues; compared to the major enamel protein amelogenin, cementum proteins were low in proline, histidine and methionine. The human and mouse putative intermediate cementum proteins appear to represent a distinct class of enamel-related proteins. Moreover, these results support the hypothesis that epithelial root sheath epithelia express several cementum proteins immunologically related to canonical enamel proteins.

摘要

十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、免疫印迹和氨基酸组成分析应用于与釉原蛋白和釉蛋白免疫相关的人和小鼠无细胞牙骨质蛋白。在该分析中，抗小鼠釉原蛋白、抗人釉蛋白和合成肽（例如-LPPHPGHPGYIC-）抗体显示与分子量为72,000道尔顿（72 kDa）的牙冠来源釉蛋白、釉原蛋白（26 kDa）发生交叉反应，并且还与四种人牙骨质蛋白（72、58、50和26 kDa）以及两种小鼠牙骨质蛋白（72和26 kDa）发生交叉反应。每种抗体都识别牙根来源的牙骨质多肽，这些多肽与牙冠来源的釉蛋白共享一个或多个表位。牙冠来源和牙根来源的釉相关蛋白的分子量和等电点相似。人和小鼠牙骨质蛋白分析显示，其特征性氨基酸组成富含谷氨酰胺、丝氨酸、甘氨酸、丙氨酸、脯氨酸、缬氨酸和亮氨酸残基；与主要的釉蛋白釉原蛋白相比，牙骨质蛋白的脯氨酸、组氨酸和蛋氨酸含量较低。人和小鼠假定的中间牙骨质蛋白似乎代表了一类独特的釉相关蛋白。此外，这些结果支持上皮根鞘上皮表达几种与典型釉蛋白免疫相关的牙骨质蛋白的假说。