Akbal Ayla, Oğuz Sevilay, Gökmen Ferhat, Bilim Serhat, Reşorlu Hatice, Sılan Fatma, Uludağ Ahmet
Department of Physical Medicine and Rehabilitation, Çanakkale Onsekiz Mart University, Çanakkale, Turkey,
Clin Rheumatol. 2015 Feb;34(2):301-6. doi: 10.1007/s10067-014-2581-7. Epub 2014 Apr 3.
We aimed to determine in psoriatic arthritis (PsA) patients the Toll-like receptor (TLR) 4 and C-reactive gene (CRP) polymorphisms and allele frequency and to investigate the relationship between clinical parameters and gene polymorphisms. We enrolled in this study 31 PsA and 41 healthy control subjects. PsA diagnosis was according to CASPAR criteria. Bath ankylosing spondylitis diseases activity index, Maastricht ankylosing spondylitis enthesitis score, and Bath ankylosing spondylitis functional index were measured. C, A, and T alleles of CRP and A and G alleles of TLR 4 were determined using the analysis of melting curves after real-time PCR. CRP A, C, and T allele frequency in controls was 26.8, 73.2, and 36.6%, respectively. In the PsA patient group, A, C, and T allele frequency was 9.7, 87.1, and 12.9%, respectively. Between control and PsA groups, there was a significant difference in A, C, and T allele frequency (P = 0.008, 0.038, and 0.001, respectively). The frequency of CRP gene polymorphisms (CA, AA, CT, TA, and TT alleles) in the control group was 56.1% and in the PsA group was 22.6%. There was a significant difference between the two groups (P = 0.004). The absence of a CRP gene polymorphism was a risk factor for PsA (odds ratio 4.3, 95% CI; 1.5-12.4, P = 0.005). TLR gene haploid frequency was investigated, and all control subjects had the wild-type AA allele. PsA patient GA allele frequency was 6.5%. There was no significant difference between the two groups (P = 0.182). GA mutant allele frequency was related to PsA (odds ratio 7.03, 95% CI; 0.32-151.9, P = 0.214). We have shown that CRP gene polymorphisms are higher in control subjects than PsA patients, and TLR 4 gene polymorphisms were found to be related to PsA.
我们旨在确定银屑病关节炎（PsA）患者中Toll样受体（TLR）4和C反应蛋白基因（CRP）的多态性及等位基因频率，并研究临床参数与基因多态性之间的关系。本研究纳入了31例PsA患者和41例健康对照者。PsA的诊断依据CASPAR标准。测量了巴斯强直性脊柱炎疾病活动指数、马斯特里赫特强直性脊柱炎附着点炎评分和巴斯强直性脊柱炎功能指数。采用实时PCR后熔解曲线分析确定CRP的C、A和T等位基因以及TLR 4的A和G等位基因。对照组中CRP A、C和T等位基因频率分别为26.8%、73.2%和36.6%。在PsA患者组中，A、C和T等位基因频率分别为9.7%、87.1%和12.9%。对照组和PsA组之间，A、C和T等位基因频率存在显著差异（P分别为0.008、0.038和0.001）。对照组中CRP基因多态性（CA、AA、CT、TA和TT等位基因）的频率为56.1%，PsA组为22.6%。两组之间存在显著差异（P = 0.004）。CRP基因多态性的缺失是PsA的一个危险因素（比值比4.3，95%可信区间；1.5 - 12.4，P = 0.005）。研究了TLR基因单倍型频率，所有对照者均有野生型AA等位基因。PsA患者中GA等位基因频率为6.5%。两组之间无显著差异（P = 0.182）。GA突变等位基因频率与PsA相关（比值比7.03，95%可信区间；0.32 - 151.9，P = 0.214）。我们已经表明，对照组中CRP基因多态性高于PsA患者，并且发现TLR 4基因多态性与PsA相关。
