Akbal Ayla, Reşorlu Hatice, Gökmen Ferhat, Savaş Yılmaz, Zateri Çoşkun, Sargin Betül, Bozkurt Emre, Sılan Fatma, Özdemir Öztürk
Department of Physical Medicine and Rehabilitation, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
Department of Medical Genetics, Çanakkale Onsekiz Mart University, Çanakkale, Turkey.
Int J Rheum Dis. 2016 Jan;19(1):43-8. doi: 10.1111/1756-185X.12619. Epub 2015 Oct 20.
Previous studies have shown that C-reactive protein (CRP) gene polymorphism can be related to inflammatory changes. The present study aimed to examine the association between CRP gene polymorphism and clinical and laboratory findings in ankylosing spondylitis (AS) patients.
A total of 80 patients, 40 with AS and 40 controls, were included in the study. Diagnosis of AS was made according to Assessment in AS International Working Group criteria. Bath Ankylosing Spondylitis Disease Activity Index, Bath Ankylosing Spondylitis Functional Index, Bath Ankylosing Spondylitis Metrology Index and Bath Ankylosing Spondylitis Radiology Index scores were evaluated. CRP gene C, A and T alleles were evaluated and were determined using the analysis of melting curves after real time polymerase chain reaction. The odds ratios were calculated for all alleles and haploids of the CRP gene. We investigated the relationship between the CRP polymorphism and clinical and laboratory findings.
A, C, T allele frequencies in the control group were 15%, 57.5% and 27.5%. The allele frequencies in the AS group were 38%, 68.8% and 26.2%. While C and T allele frequencies were shown to be similar in the two groups, A allele frequency was higher in the AS group compared to the control group. The CC wild allele was 42.5% in the control group and 47.5% in the AS group (P = 1.0). Odds ratios for the C allele were 1.6, for the CC haploid 1.2 and for the CT haploid 3.7. Chest expansion and finger-to-ground distance was better in the CRP gene polymorphism group compared to the no polymorphism group.
The presence of the CRP gene CC wild haploid and C allele in patients may indicate an increased risk for AS.
既往研究表明，C反应蛋白（CRP）基因多态性可能与炎症变化有关。本研究旨在探讨CRP基因多态性与强直性脊柱炎（AS）患者临床及实验室检查结果之间的关联。
本研究共纳入80例患者，其中40例AS患者和40例对照。AS的诊断依据国际AS工作组标准进行评估。评估了巴斯强直性脊柱炎疾病活动指数、巴斯强直性脊柱炎功能指数、巴斯强直性脊柱炎测量指数和巴斯强直性脊柱炎放射学指数评分。对CRP基因的C、A和T等位基因进行评估，并在实时聚合酶链反应后通过熔解曲线分析进行测定。计算了CRP基因所有等位基因和单倍型的比值比。我们研究了CRP基因多态性与临床及实验室检查结果之间的关系。
对照组中A、C、T等位基因频率分别为15%、57.5%和27.5%。AS组的等位基因频率分别为38%、68.8%和26.2%。虽然两组的C和T等位基因频率相似，但AS组的A等位基因频率高于对照组。对照组中CC野生型等位基因占42.5%，AS组中占47.5%（P = 1.0）。C等位基因的比值比为1.6，CC单倍型为1.2，CT单倍型为3.7。与无基因多态性组相比，CRP基因多态性组的胸廓活动度和指地距更好。
患者中存在CRP基因CC野生型单倍型和C等位基因可能提示AS风险增加。
