针对由[甲硫氨酸] - 脑啡肽互补RNA指定的肽的单克隆抗体识别δ类阿片受体。
Monoclonal antibody against a peptide specified by [Met]-enkephalin complementary RNA recognizes the delta-class opioid receptor.
作者信息
Carr D J, Blalock J E, Bost K L
机构信息
Department of Physiology and Biophysics, University of Alabama, Birmingham 35294.
出版信息
Immunol Lett. 1989 Feb;20(3):181-6. doi: 10.1016/0165-2478(89)90077-1.
Monoclonal antibodies (MAB) were developed which recognize a peptide, His-Glu-Ala-Pro-Ile (HEAPI), encoded by the RNA complementary to the mRNA specifying [Met]-enkephalin. One such MAB (designated 6193) exhibited a high degree of reactivity to the peptide sequence. Other characteristics of 6193 MAB include: the ability to block opioid ligand binding in a radioreceptor assay; agonist activity similar to opioid peptides in suppressing cAMP production; and the recognition of a 58 kDa protein on the surface of the neuroblastoma x glioma cell line, NG108-15. These results are consistent with a reactivity of 6193 MAB with the delta-class opioid receptor.
开发出了单克隆抗体（MAB），这些抗体能够识别一种由与指定[甲硫氨酸]脑啡肽的mRNA互补的RNA编码的肽，即组氨酸-谷氨酸-丙氨酸-脯氨酸-异亮氨酸（HEAPI）。其中一种这样的MAB（命名为6193）对该肽序列表现出高度反应性。6193 MAB的其他特性包括：在放射受体测定中阻断阿片样物质配体结合的能力；在抑制环磷酸腺苷（cAMP）产生方面与阿片样肽类似的激动剂活性；以及识别神经母细胞瘤×胶质瘤细胞系NG108-15表面的一种58 kDa蛋白质。这些结果与6193 MAB与δ类阿片受体的反应性一致。
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