维德曼春黄菊的脂肪酸组成及临床前研究：一种新型抗炎剂的发现
Fatty acid composition and preclinical resarches on Anthemis wiedemanniana Fisch. & Mey.: Discovery of a new anti-inflammatory agent.
作者信息
Gönenç Tuba Mert, Akkol Esra Küpeli, Süntar Ipek, Erdoğan Tuğçe Fafal, Kıvçak Bijen
机构信息
Department of Pharmacognosy, Ege University, 35100 Izmir, Turkey.
Department of Pharmacognosy, Gazi University, Etiler 06330, Ankara, Turkey.
出版信息
Pharmacogn Mag. 2014 Jan;10(37):53-60. doi: 10.4103/0973-1296.126660.
BACKGROUND
Anthemis species have been used for the treatment of gastrointestinal disorders, hemorrhoid, stomachache and inflammatory diseases in Turkish folk medicine. Anthemis wiedemanniana Fisch. And Mey. is an endemic plant used as painkiller, antispasmodic, sedative and for the treatment of urinary inflammations.
OBJECTIVE
The objective of the present study is to evaluate the anti-inflamatory activity of the extracts of A. wiedemanniana by using in vivo methods.
MATERIALS AND METHODS
Carrageenan-, PGE2- and serotonin-induced hind paw edema, 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema and acetic acid-induced increase in capillary permeability models were used for the anti-inflammatory activity assessment. Moreover, the fatty acid composition of A. wiedemanniana was investigated by gas chromatography (GC).
RESULTS
n-Hexane, diethyl ether and total sesquiterpene lactone extracts exhibited significant inhibition in carrageenan-induced hind paw edema and acetic acid-induced increase in capillary permeability model. n-Hexane and total sesquiterperne lactone extracts showed anti-inflammatory activity in PGE2- and serotonin-induced hind paw edema model. However none of the extracts displayed significant activity in TPA-induced ear edema model in mice. C4:0 (Buthyric acid), C20:0 (Arachidic acid) and C16:1 (Palmitoleik acid) were found to be the major fatty acids in these species. Saturated fatty acids (SFA) were found in higher amounts than monounsaturated fatty acids and polyunsaturated fatty acids. SFAs were determined as 63.17%, UFAs as 20.89% and PuFAs as 15.95%.
CONCLUSION
This study confirms the traditional usage of A. wiedemanniana for inflammatory diseases.
背景
在土耳其民间医学中，春黄菊属植物已被用于治疗胃肠道疾病、痔疮、胃痛和炎症性疾病。维德曼春黄菊（Anthemis wiedemanniana Fisch. And Mey.）是一种本土植物，用作止痛药、解痉药、镇静剂，并用于治疗泌尿炎症。
目的
本研究的目的是通过体内实验方法评估维德曼春黄菊提取物的抗炎活性。
材料与方法
采用角叉菜胶、前列腺素E2和血清素诱导的后爪水肿、12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的小鼠耳水肿以及乙酸诱导的毛细血管通透性增加模型来评估抗炎活性。此外，通过气相色谱法（GC）研究了维德曼春黄菊的脂肪酸组成。
结果
正己烷、乙醚和总倍半萜内酯提取物在角叉菜胶诱导的后爪水肿和乙酸诱导的毛细血管通透性增加模型中表现出显著抑制作用。正己烷和总倍半萜内酯提取物在前列腺素E2和血清素诱导的后爪水肿模型中显示出抗炎活性。然而，在TPA诱导的小鼠耳水肿模型中，没有一种提取物表现出显著活性。发现丁酸（C4:0）、花生酸（C20:0）和棕榈油酸（C16:1）是这些物种中的主要脂肪酸。饱和脂肪酸（SFA）的含量高于单不饱和脂肪酸和多不饱和脂肪酸。饱和脂肪酸含量为63.17%，不饱和脂肪酸为20.89%，多不饱和脂肪酸为15.95%。
结论
本研究证实了维德曼春黄菊在治疗炎症性疾病方面的传统用途。
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