Dong Ning, Di Zhang, Yu Yong, Yuan Meng, Zhang Xiaohua, Li Huirong
Wei Sheng Wu Xue Bao. 2013 Dec 4;53(12):1295-306.
To investigate the composition of culturable bacteria from soil of Grove Mountains, East Antarctica and analyze the production of extracellular hydrolytic enzymes and antibacterial and antifugal activities of these culturable bacteria.
We used spread plate method to obtain culturable bacteria. Phylogenetic relationship was analysed based on their 16S rRNA gene sequences. We used plate method and agar block method to detect the production of extracellular enzymes and antibacterial and antifungal activities of these bacteria, separately.
We obtained a total of 39 isolates from all the soil samples. They belonged to 20 genera and grouped into Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, Deinococcus-Thermus lineages, of which each group occupied 48.7%, 25.6%, 20.5%, 2.6%, 2.6% of the total, respectively. Bacillus was dominant. We isolated different strains from soil stored at different temperatures, and this may be explained as viable bacteria were different at diverse temperatures. Of the 39 isolates 33 showed the activity of producing at least one extracellular enzyme. Amylase-producing strains were the most (64.1%), 6 strains were able to inhibit the growth of at least one tested bacterium or fungus.
Strains with extracellular enzyme activity and antibacterial and antifungal activity would contribute to the exploration of cold-active enzymes and antibacterial (antifungal) material in Antarctica.
研究东南极格罗夫山土壤中可培养细菌的组成，并分析这些可培养细菌胞外水解酶的产生情况以及抗菌和抗真菌活性。
采用涂布平板法获取可培养细菌。基于其16S rRNA基因序列分析系统发育关系。分别采用平板法和琼脂块法检测这些细菌胞外酶的产生情况以及抗菌和抗真菌活性。
从所有土壤样品中共获得39株分离菌。它们属于20个属，分为厚壁菌门、变形菌门、放线菌门、拟杆菌门、嗜热栖热放线菌谱系，每组分别占总数的48.7%、25.6%、20.5%、2.6%、2.6%。芽孢杆菌属占主导。我们从不同温度保存的土壤中分离出不同菌株，这可能是因为不同温度下存活的细菌不同。在39株分离菌中，33株表现出产生至少一种胞外酶的活性。产淀粉酶的菌株最多（64.1%），6株能够抑制至少一种受试细菌或真菌的生长。
具有胞外酶活性以及抗菌和抗真菌活性的菌株将有助于在南极探索冷活性酶和抗菌（抗真菌）物质。
