Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Syst Appl Microbiol. 2011 Jul;34(5):360-7. doi: 10.1016/j.syapm.2011.02.002. Epub 2011 Apr 17.
We studied the culturable heterotrophic bacterial diversity present at the site of the new Princess Elisabeth Station at Utsteinen (Dronning Maud Land, East Antarctica) before construction. About 800 isolates were picked from two terrestrial microbial mat samples after incubation on several growth media at different temperatures. They were grouped using rep-PCR fingerprinting and partial 16S rRNA gene sequencing. Phylogenetic analysis of the complete 16S rRNA gene sequences of 93 representatives showed that the isolates belonged to five major phyla: Actinobacteria, Bacteroidetes, Proteobacteria, Firmicutes and Deinococcus-Thermus. Isolates related to the genus Arthrobacter were the most prevalent whereas the genera Hymenobacter, Deinococcus, Cryobacterium and Sphingomonas were also recovered in high numbers in both samples. A total of 35 different genera were found, the majority of which has previously been reported from Antarctica. For the genera Aeromicrobium, Aurantimonas, Rothia, Subtercola, Tessaracoccus and Xylophilus, this is the first report in Antarctica. In addition, numerous potential new species and new genera were recovered; many of them currently restricted to Antarctica, particularly in the phyla Bacteroidetes and Deinococcus-Thermus.
我们在新的伊丽莎白公主站（位于东南极洲的默德皇后地）建设之前，研究了乌特施泰因（Utsteinen）该地点存在的可培养异养细菌多样性。在不同温度下的几种生长培养基上孵育后，从两个陆地微生物垫样品中挑出了约 800 个分离株。使用重复序列 PCR 指纹图谱和部分 16S rRNA 基因测序对它们进行分组。对 93 个代表物的完整 16S rRNA 基因序列的系统发育分析表明，分离株属于五个主要门：放线菌门、拟杆菌门、变形菌门、厚壁菌门和 Deinococcus-Thermus 门。与节杆菌属相关的分离株最为普遍，而海姆霍伯菌属、Deinococcus 属、嗜冷杆菌属和鞘氨醇单胞菌属在两个样品中也大量回收。共发现 35 个不同的属，其中大多数以前曾在南极洲报道过。对于 Aeromicrobium、Aurantimonas、Rothia、Subtercola、Tessaracoccus 和 Xylophilus 属，这是在南极洲的首次报道。此外，还回收了许多潜在的新物种和新属；其中许多目前仅限于南极洲，特别是在拟杆菌门和 Deinococcus-Thermus 门。
