He Qiao, Ding Chen, Li Guizhong, Cheng Hao, Cheng Lei, Zhang Hui
Wei Sheng Wu Xue Bao. 2013 Dec 4;53(12):1307-17.
To evaluate coal bed methane production potential and characterize the in situ microbial communities of coal bed.
Coal samples were incubated under anaerobic conditions: mimicking coal bed condition, supplementing with methanogenic hydrocarbon degrading consortium, or adding with exogenetic substrate. Methane production was observed over time using gas chromatograph, and the in situ bacterial and archaeal communities were revealed using pyrosequencing.
Enrichment incubation revealed that 3 of total 10 coal samples microcosms produced methane; bioaugmentation and substrate addition could enhance methane production of coal sample HF. Hydrogenotrophic Methanoculleus and acetoclastic Methanosaeta dominated the archaeal community of coal sample SL, while the bacterial domain was mainly composed of Firmicutes (54.4%), Proteobacteria (30.9%), uncultured bacteria (10.8%), Caldiserica (1.5%) and Thermotogae (1.3%).
The methane production potential of coal bed samples with different maturity is different; the in situ coal bed microcosms are likely involved in hydrocarbons degradation and methane production.
评估煤层气的生产潜力并表征煤层原位微生物群落。
在厌氧条件下培养煤样：模拟煤层条件、补充产甲烷烃降解菌群或添加外源底物。使用气相色谱仪随时间观察甲烷生成情况，并通过焦磷酸测序揭示原位细菌和古菌群落。
富集培养表明，10个煤样微观世界中有3个产生了甲烷；生物强化和添加底物可提高煤样HF的甲烷产量。氢营养型甲烷袋菌属和乙酸营养型甲烷八叠球菌属在煤样SL的古菌群落中占主导地位，而细菌域主要由厚壁菌门（54.4%）、变形菌门（30.9%）、未培养细菌（10.8%）、暖绳菌纲（1.5%）和栖热袍菌门（1.3%）组成。
不同成熟度的煤层样品的甲烷生产潜力不同；原位煤层微观世界可能参与了烃类降解和甲烷生成。
