Lacy M J, Dombrink-Kurtzman M A, Voss E W
Department of Microbiology, University of Illinois, Urbana 61801.
J Immunol. 1989 May 15;142(10):3482-8.
Polyclonal immune network antibodies were quantitated and characterized in a syngeneic BALB/c murine system. Immunizations of BALB/c antifluorescein mAb 9-40 conjugated to keyhole limpet hemocyanin, produced anti-Id (anti-9-40, 39 to 190 micrograms/ml) as well as anti-fluorescein (anti-Fl, 12 to 109 micrograms/ml). Separately, immunizations of polyclonal anti-9-40, developed significant anti-Fl serum levels in the secondary (2 degrees) response (50 to 270 micrograms/ml), which decreased in the 3 degrees response (50 to 180 micrograms/ml) and thereafter, although levels of 9-40 idiotypically related antibodies increased. Polyclonal 2 degrees anti-anti-9-40 exhibited variant anti-Fl active sites, was antigenically more cross-reactive than polyclonal 2 degrees anti-Fl, but did not exhibit affinity maturation for fluorescein relative to 1 degrees anti-anti-9-40. In addition, the 9-40 idiotype constituted a small (less than 1.0%) percent of the 2 degrees and 3 degrees anti-Fl (ab1) immune response. When viewed within the context of an antigenic system that possesses widely diverse idiotypy, continued introduction of polyclonal anti-Id appears eventually to: 1) induce polyclonal ab3 with quantitative expression of idiotypically related antibodies in preference to ab3 of ancestral (9-40) antigenic specificity, 2) relative to ab1, induce a 100-fold increase in the level of ab3 antibodies that have both ancestral idiotype and ancestral antigen reactivity, and 3) induce polyclonal ab3 antibodies with a measurably wider range of antigenic reactivities than those of polyclonal ab1. These quantitative data may reflect the natural state of an immune network in a diverse antigenic response.
在同基因BALB/c小鼠系统中对多克隆免疫网络抗体进行了定量和表征。用与钥孔戚血蓝蛋白偶联的BALB/c抗荧光素单克隆抗体9-40进行免疫，产生了抗独特型抗体（抗9-40，39至190微克/毫升）以及抗荧光素抗体（抗Fl，12至109微克/毫升）。另外，用多克隆抗9-40进行免疫，在二级（2°）反应中产生了显著水平的抗Fl血清（50至270微克/毫升），在三级反应中该水平下降（50至180微克/毫升），此后虽有所下降，但9-40独特型相关抗体的水平有所增加。多克隆二级抗抗9-40表现出可变的抗Fl活性位点，在抗原性上比多克隆二级抗Fl具有更多的交叉反应性，但相对于一级抗抗9-40，对荧光素未表现出亲和力成熟。此外，9-40独特型在二级和三级抗Fl（ab1）免疫反应中所占比例很小（小于1.0%）。在具有广泛多样独特型的抗原系统背景下观察时，持续引入多克隆抗独特型抗体最终似乎会：1）诱导多克隆ab3，优先定量表达独特型相关抗体而非具有祖先（9-40）抗原特异性的ab3；2）相对于ab1，诱导具有祖先独特型和祖先抗原反应性的ab3抗体水平增加100倍；3）诱导多克隆ab3抗体，其抗原反应性范围比多克隆ab1的更宽。这些定量数据可能反映了多样化抗原反应中免疫网络的自然状态。
