Docheva Margarita, Dagnon Soleya, Statkova-Abeghe Stela
a Department of Tobacco Chemistry , Tobacco and Tobacco Products Institute , Plovdiv , Bulgaria.
Nat Prod Res. 2014;28(17):1328-34. doi: 10.1080/14786419.2014.902947. Epub 2014 Apr 4.
The flavonoid-rich natural products exert a wide range of pharmacological properties. This investigation aimed at obtaining extracts from tobacco cultivars and waste enriched with flavonoids and purified from nicotine and phenolic acids, and evaluating their radical scavenging potential. Extraction with a mixture of ethyl acetate-methanol (1:1, v/v) was employed resulting in 100% yield for flavonoids and 36% yield for phenolic acids. The crude extracts were purified using preparative column chromatography on silica gel. The content of flavonoids in the purified extracts varied from 8.8 ± 1.1% to 14.3 ± 1.8%. Nicotine was not detected in amounts higher than 0.3 μg mL(-1). The content of phenolic acids was lower than 1.0%. The radical scavenging potential of extracts from tobacco cultivars exhibited values from IC50 = 35.0 ± 3.1 to 64.6 ± 7.5 μg mL(-1). The extracts obtained by the proposed procedure are enriched with flavonoids with high radical scavenging potential and are purified from nicotine and phenolic acids. They can be regarded as potential biopharmaceuticals.
富含黄酮类化合物的天然产物具有广泛的药理特性。本研究旨在从烟草品种和废弃物中获取富含黄酮类化合物且不含尼古丁和酚酸的提取物，并评估其自由基清除潜力。采用乙酸乙酯 - 甲醇（1:1，v/v）混合物进行提取，黄酮类化合物的得率为100%，酚酸的得率为36%。粗提取物通过硅胶柱制备色谱法进行纯化。纯化提取物中黄酮类化合物的含量在8.8 ± 1.1%至14.3 ± 1.8%之间变化。未检测到尼古丁含量高于0.3 μg mL(-1)。酚酸含量低于1.0%。烟草品种提取物的自由基清除潜力表现为IC50值在35.0 ± 3.1至64.6 ± 7.5 μg mL(-1)之间。通过所提出的方法获得的提取物富含具有高自由基清除潜力的黄酮类化合物，且不含尼古丁和酚酸。它们可被视为潜在的生物药物。
