Chuba P J, Bock R, Graf G, Adam T, Göbel U
Institut für Medizinische Mikrobiologie und Hygiene der Universität Freiburg, FRG.
J Gen Microbiol. 1988 Jul;134(7):1923-30. doi: 10.1099/00221287-134-7-1923.
The taxonomy of the family Pasteurellaceae has remained controversial despite investigations of biochemistry, serology, and nucleic acid relatedness. In an attempt to resolve some of this confusion, we have partially sequenced the 16S rRNAs of seven members of the family, representing all three genera. The sequences were aligned, similarity scores calculated, and single, average and complete linkage cluster analysis of the resulting distance matrix performed. In this way, an evolutionary branching pattern of these closely related species was reconstructed, and the approximate phylogenetic position of the family determined. Actinobacillus (Haemophilus) actinomycetemcomitans clustered with Haemophilus instead of Actinobacillus, supporting transfer of this species to the genus Haemophilus. Thus cluster analysis of phylogenetic relatedness was found to be particularly useful for studying closely related organisms, and could be performed using a microcomputer.
尽管对巴氏杆菌科进行了生物化学、血清学及核酸相关性研究，但其分类学仍存在争议。为解决部分此类混淆问题，我们对该科七个成员的16S rRNA进行了部分测序，这七个成员代表了所有三个属。将序列进行比对，计算相似性得分，并对所得距离矩阵进行单链、平均链和完全连锁聚类分析。通过这种方式，重建了这些密切相关物种的进化分支模式，并确定了该科大致的系统发育位置。伴放线放线杆菌（嗜血性放线杆菌）与嗜血杆菌属聚类，而非与放线杆菌属聚类，支持将该物种转移至嗜血杆菌属。因此，发现系统发育相关性的聚类分析对于研究密切相关的生物体特别有用，并且可以使用微型计算机进行。
