不可分型流感嗜血杆菌的长片段PCR核糖体分型
Long PCR-ribotyping of nontypeable Haemophilus influenzae.
作者信息
Smith-Vaughan H C, Sriprakash K S, Mathews J D, Kemp D J
机构信息
Menzies School of Health Research, Darwin, Northern Territory, Australia.
出版信息
J Clin Microbiol. 1995 May;33(5):1192-5. doi: 10.1128/jcm.33.5.1192-1195.1995.
Abstract
PCR-ribotyping, a new typing method based on long PCR, has been developed for nontypeable Haemophilus influenzae (NTHi). Ribosomal operons of NTHi were amplified by long PCR and were found to be highly polymorphic for internal HaeIII sites. The technique was applied to 49 isolates previously subjected to conventional ribotyping, and the two methods showed a high level of concordance for serial isolates from individual subjects. PCR-ribotyping provides a powerful new typing tool for strain characterization in epidemiological investigations of NTHi.
摘要
基于长片段PCR的新型分型方法——PCR核糖体分型已被开发用于不可分型流感嗜血杆菌（NTHi）。通过长片段PCR扩增NTHi的核糖体操纵子，发现其内部HaeIII位点具有高度多态性。该技术应用于49株先前进行过传统核糖体分型的菌株，两种方法对来自个体受试者的系列菌株显示出高度一致性。PCR核糖体分型为NTHi流行病学调查中的菌株特征分析提供了一种强大的新型分型工具。
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