Hamada Toshiyuki, Niki Tomoko, Ishida Norio
Applied Molecular-Imaging Physics, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Japan; Ishida Group of Clock Gene, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.
Genes Cells. 2014 May;19(5):441-8. doi: 10.1111/gtc.12144. Epub 2014 Apr 4.
p53 protein plays a role for control of cell proliferation and the induction of apoptosis in normal cells. However, its role in the circadian rhythms that control many physiological functions including locomotor behavior remains unknown. The present study examined the locomotors activity rhythms of mice which have homozygous mutations of p53 gene. The period of drinking activity rhythms in p53 knockout (p53 KO) mice became unstable under constant dark. Light pulse causes a big phase shifts at CT15.5-17, when p53 mRNA expression peaks in the suprachiasmatic nucleus (SCN). Furthermore, photic entrainment of p53 KO mice is unusual under light-dark conditions. These findings suggest that p53 is involved in entrainment of the circadian behavioral rhythm.
p53蛋白在正常细胞中对控制细胞增殖和诱导细胞凋亡发挥作用。然而，其在控制包括运动行为在内的许多生理功能的昼夜节律中的作用尚不清楚。本研究检测了具有p53基因纯合突变的小鼠的运动活动节律。在持续黑暗条件下，p53基因敲除（p53 KO）小鼠的饮水活动节律周期变得不稳定。光脉冲在CT15.5 - 17时会引起较大的相位偏移，此时视交叉上核（SCN）中p53 mRNA表达达到峰值。此外，在明暗条件下，p53 KO小鼠的光诱导同步是异常的。这些发现表明p53参与了昼夜行为节律的同步。
