Molecular Biology, Faculty of Medicine, Titu Maiorescu University, Bucharest, Romania.
Constantin Brancusi University, Faculty of Medical Science and Behaviors, Târgu Jiu, Romania.
Biomed Pharmacother. 2018 Oct;106:50-53. doi: 10.1016/j.biopha.2018.06.072. Epub 2018 Jun 23.
In the past few years has used thetechnique for analyzing deletions of genes, its rearrangements, cross-reactivity or multiplications in human genome affected of genetic diseases. Was proved that, the best techniques in the investigation of malignant lymphocytes are the Flow Cytometry, Elisa, ICT and Fluorescence in situ hybridization (FISH). Last method, FISH is used as an alternative to chromosomal banding, a conventional application in molecular medicine and can detect the chromosomal rearrangements and complexes of different genes in malignant diseases, like chronic lymphocytic leukemia (CLL), acute lymphocytic leukemia, (ALL), or multiple myeloma (MM). Identification of P53 gene deletions and mutations in regions of chromosome 17 in hematological malignancies is important because these mutations have an impact on the clinical management of patients.
在过去的几年中，该技术已被用于分析人类基因组中基因的缺失、重排、交叉反应或扩增，以研究遗传疾病。已经证明，在恶性淋巴细胞研究中最好的技术是流式细胞术、ELISA、ICT 和荧光原位杂交（FISH）。最后一种方法，FISH 可作为染色体带分析的替代方法，这是分子医学中的一种常规应用，可检测恶性疾病中的染色体重排和不同基因的复合物，如慢性淋巴细胞白血病（CLL）、急性淋巴细胞白血病（ALL）或多发性骨髓瘤（MM）。在血液系统恶性肿瘤中鉴定 17 号染色体区域的 P53 基因突变和缺失很重要，因为这些突变会影响患者的临床管理。
