Zatsiorskaia S L
Lab Delo. 1989(3):15-8.
The author compares the potentialities of several nutrient media for the isolation of Group B streptococci. The media used have been as follows: agar, manufactured by the Daghestan Research Institute for Nutrient Media, with 5% of human defibrinated blood (HBA), and blood agar containing 5% of sheep red blood cells (BAS). Gentamicin (8 micrograms/ml) and nalidixic acid (15 micrograms/ml) have been added into the media selectively, and the media have been then labeled as SHBA or SBAS. Liquid nutrient medium: placental broth with the same selective agents in the same concentrations (PBS), prepared by the author, has also been employed. 213 lochia samples and 196 urine samples from pregnant women have been examined. Urine cultivation in PBS has yielded a 4-fold increase (p less than 0.01) of the isolation rate of streptococci vs. other media. The difference in the isolation rates of streptococci cultivated in HBA and SHBA has been insignificant. Addition of donor or sheep red cells to solid nutrient media has provided similar isolation rates of streptococci. The best results have been achieved when liquid selective medium and blood agar have been simultaneously employed.
作者比较了几种用于分离B族链球菌的营养培养基的潜力。所使用的培养基如下：由达吉斯坦营养培养基研究所生产的含有5%人去纤维蛋白血的琼脂（HBA），以及含有5%绵羊红细胞的血琼脂（BAS）。已将庆大霉素（8微克/毫升）和萘啶酸（15微克/毫升）选择性地添加到培养基中，然后将这些培养基标记为SHBA或SBAS。液体营养培养基：作者制备的含有相同浓度相同选择性试剂的胎盘肉汤（PBS）也已被使用。对213份孕妇恶露样本和196份孕妇尿液样本进行了检测。与其他培养基相比，在PBS中进行尿液培养时，链球菌的分离率提高了4倍（p<0.01）。在HBA和SHBA中培养的链球菌分离率差异不显著。向固体营养培养基中添加供体或绵羊红细胞可得到相似的链球菌分离率。当同时使用液体选择性培养基和血琼脂时，取得了最佳结果。
