Albers A C, Sniffen J M, Freedel D N, Matuscak R R, Davis L, Benko B, Raneri S
Am J Med Technol. 1983 Nov;49(11):807-11.
Increased awareness of the importance of Streptococcus agalactiae (Group B streptococcus) in neonatal morbidity and mortality has demonstrated the need for a selective and differential primary plating medium. In this report we describe a selective pigment medium containing colistin and nalidixic acid that allows presumptive identification of S. agalactiae from the primary plate, even when specimens are polymicrobic. A preliminary study compared the value of three media for pigment production by 137 known S. agalactiae strains. This was followed by a clinical laboratory trial using the best of these pigment media at an obstetric hospital in which 581 specimens from neonates, female reproductive tracts, and urinary tracts were screened. Subsequent to the clinical laboratory trial, an epidemiological investigation using the selective pigment medium was conducted on a mother/infant population. Of the 1,331 specimens screened for S. agalactiae, 75 (5.6%) were positive on 5% sheep blood agar and 79 (5.9%) were positive on the selective pigment medium.
对无乳链球菌（B族链球菌）在新生儿发病和死亡中的重要性认识的提高，表明需要一种选择性和鉴别性的初代接种培养基。在本报告中，我们描述了一种含有黏菌素和萘啶酸的选择性色素培养基，即使标本是多菌混合的，也能从初代平板上初步鉴定出无乳链球菌。一项初步研究比较了三种培养基对137株已知无乳链球菌菌株产生色素的价值。随后在一家产科医院进行了一项临床实验室试验，使用这些色素培养基中效果最好的一种，对581份来自新生儿、女性生殖道和泌尿道的标本进行筛查。临床实验室试验之后，对母婴群体进行了一项使用选择性色素培养基的流行病学调查。在筛查的1331份无乳链球菌标本中，75份（5.6%）在5%羊血琼脂上呈阳性，79份（5.9%）在选择性色素培养基上呈阳性。
