Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Milan, Italy; Laboratory of Technical Medical Sciences, Department of Biomedical Science for Health, University of Milan, Milan, Italy.
APMIS. 2014 Oct;122(10):1013-9. doi: 10.1111/apm.12254. Epub 2014 Apr 4.
To address the problem of limited efficacy of existing antibiotics in the treatment of bacterial biofilm, it is necessary to find alternative remedies. One candidate could be hyaluronic acid; this study therefore aimed to evaluate the in vitro antiadhesive and antibiofilm activity of hyaluronic acid toward bacterial species commonly isolated from respiratory infections. Interference exerted on bacterial adhesion was evaluated by using Hep-2 cells, while the antibiofilm activity was assessed by means of spectrophotometry after incubation of biofilm with hyaluronic acid and staining with crystal violet. Our data suggest that hyaluronic acid is able to interfere with bacterial adhesion to a cellular substrate in a concentration-dependent manner, being notably active when assessed as pure substance. Moreover, we found that Staphylococcus aureus biofilm was more sensitive to the action of hyaluronic acid than biofilm produced by Haemophilus influenzae and Moraxella catarrhalis. In conclusion, hyaluronic acid is characterized by notable antiadhesive properties, while it shows a moderate activity against bacterial biofilm. As bacterial adhesion to oral cells is the first step for colonization, these results further sustain the role of hyaluronic acid in prevention of respiratory infections.
为了解决现有抗生素在治疗细菌生物膜方面疗效有限的问题，需要寻找替代疗法。候选药物之一可能是透明质酸；因此，本研究旨在评估透明质酸对呼吸道感染常见分离菌的体外抗黏附及抗生物膜活性。通过 Hep-2 细胞评估对细菌黏附的干扰作用，通过孵育生物膜并用结晶紫染色后分光光度法评估抗生物膜活性。我们的数据表明，透明质酸能够以浓度依赖的方式干扰细菌对细胞底物的黏附，作为纯物质评估时表现出显著的活性。此外，我们发现金黄色葡萄球菌生物膜对透明质酸的作用比流感嗜血杆菌和卡他莫拉菌生物膜更敏感。总之，透明质酸具有显著的抗黏附特性，而对细菌生物膜表现出中等活性。由于细菌黏附到口腔细胞是定植的第一步，这些结果进一步支持了透明质酸在预防呼吸道感染中的作用。
