United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan; Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa, Japan.
Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa, Japan.
Nutrition. 2014 May;30(5):575-83. doi: 10.1016/j.nut.2013.09.017. Epub 2013 Oct 20.
Crude ethanolic extract of Peucedanum japonicum Thunb (PJT) has been identified as an antiobesity and antidiabetic candidate. The aim of this study was to assess the mechanisms related to the antiobesity activity of partially purified PJT ethanol extract in vitro.
PJT ethanol extract (EE) was partitioned into hexane phase (HP) and residual water phase. The effect of PJT extracts on triacylglycerol (TG) content, lipid metabolism-related gene expression, and energy expenditure was assessed in vitro in 3T3-L1, HepG2, and C2C12 cells. Furthermore, the active components in PJT extracts were partially profiled by high-performance liquid chromatography (HPLC) analysis.
The HP significantly down-regulated lipogenic gene expressions in hepatocytes, inhibited TG accumulation, and decreased the size of 3T3-L1 adipocytes. Moreover, the inhibition of lipid accumulation was at maximum during the adipocyte maturation stage. Furthermore, we found an increase in fat hydrolysis by HP in 3T3-L1. In C2C12 myotubes, the HP tended to enhance energy expenditure. HPLC analysis demonstrated that hydrophobic compounds available in the HP were responsible for antiobesity, which corresponded to the latter peaks on the HPLC chromatogram of EE.
Our study demonstrated that the HP plays a crucial role in regulating lipid metabolism-related gene expressions and energy expenditure in vitro. The results thus provide insight on the activity of HP involved in suppressing obesity and its chemical entities.
已鉴定出中药防风的粗乙醇提取物是一种具有减肥和抗糖尿病作用的候选药物。本研究旨在评估部分纯化的防风乙醇提取物在体外的减肥活性相关机制。
将防风乙醇提取物（EE）分配到正己烷相（HP）和残留水相。在 3T3-L1、HepG2 和 C2C12 细胞中评估防风提取物对三酰基甘油（TG）含量、脂质代谢相关基因表达和能量消耗的影响。此外，通过高效液相色谱（HPLC）分析部分分析防风提取物中的活性成分。
HP 显著下调肝细胞中的生脂基因表达，抑制 TG 积累，并减小 3T3-L1 脂肪细胞的大小。此外，在脂肪细胞成熟阶段，抑制脂质积累的作用最大。此外，我们发现 HP 增加了 3T3-L1 中的脂肪水解。在 C2C12 肌管中，HP 倾向于增加能量消耗。HPLC 分析表明，HP 中存在的疏水性化合物是其具有减肥作用的原因，这与 EE 的 HPLC 色谱图上的后峰相对应。
本研究表明，HP 在调节体外脂质代谢相关基因表达和能量消耗方面发挥着重要作用。研究结果为抑制肥胖的 HP 活性及其化学实体提供了依据。
