醛糖还原酶基因C（-106）T多态性与中国2型糖尿病患者糖尿病视网膜病变的关系

Association of C(-106)T polymorphism in aldose reductase gene with diabetic retinopathy in Chinese patients with type 2 diabetes mellitus.

作者信息

Deng Yu, Yang Xiu-fen, Gu Hong, Lim Apiradee, Ulziibat Munkhtulga, Snellingen Torkel, Xu Jun, Ma Kai, Liu Ning-pu

机构信息

Department of Ophthalmology, Fu Xing Hospital, Capital Medical University, Beijing 100038, China; 2. Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing 100730, China.

Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing 100730, China.

出版信息

Chin Med Sci J. 2014 Mar;29(1):1-6. doi: 10.1016/s1001-9294(14)60016-x.

DOI:10.1016/s1001-9294(14)60016-x

PMID:24698671

Abstract

OBJECTIVE

To identify the possible association between C(-106)T polymorphism of the aldose reductase (ALR) gene and diabetic retinopathy (DR) in a cohort of Chinese patients with type 2 diabetes mellitus (T2DM).

METHODS

From November 2009 to September 2010, patients with T2DM were recruited and assigned to DR group or diabetic without retinopathy (DWR) group according to the duration of diabetes and the grading of 7-field fundus color photographs of both eyes. Genotypes of the C(-106)T polymorphism (rs759853) in ALR gene were analyzed using the MassARRAY genotyping system and an association study was performed.

RESULTS

A total of 268 T2DM patients (129 in the DR group and 139 in the DWR group) were included in this study. No statistically significant differences were observed between the 2 groups in the age of diabetes onset (P=0.10) and gender (P=0.78). The success rate of genotyping for the study subjects was 99.6% (267/268), with one case of failure in the DR group. The frequencies of the T allele in the C(-106)T polymorphism were 16.0% (41/256) in the DR group and 19.4% (54/278) in the DWR group (P=0.36). There was no significant difference in the C(-106)T genotypes between the 2 groups (P=0.40). Compared with the wild-type genotype, odds ratio (OR) for the risk of DR was 0.7 (95% CI, 0.38-1.3) for the heterozygous CT genotype and 0.76 (95% CI, 0.18-3.25) for the homozygous TT genotype. The risk of DR was positively associated with microalbuminuria (OR=4.61; 95% CI, 2.34-9.05) and insulin therapy (OR=3.43; 95% CI, 1.94-6.09).

CONCLUSIONS

Microalbuminuria and insulin therapy are associated with the risk of DR in Chinese patients with T2DM. C(-106)T polymorphism of the ALR gene may not be significantly associated with DR in Chinese patients with T2DM.

摘要

目的

在一组中国2型糖尿病（T2DM）患者中，确定醛糖还原酶（ALR）基因C（-106）T多态性与糖尿病视网膜病变（DR）之间可能存在的关联。

方法

2009年11月至2010年9月，招募T2DM患者，并根据糖尿病病程和双眼7视野眼底彩色照片的分级，将其分为DR组或无视网膜病变糖尿病（DWR）组。使用MassARRAY基因分型系统分析ALR基因C（-106）T多态性（rs759853）的基因型，并进行关联研究。

结果

本研究共纳入268例T2DM患者（DR组129例，DWR组139例）。两组在糖尿病发病年龄（P=0.10）和性别（P=0.78）方面未观察到统计学显著差异。研究对象的基因分型成功率为99.6%（267/268），DR组有1例失败。DR组C（-106）T多态性中T等位基因频率为16.0%（41/256），DWR组为19.4%（54/278）（P=0.36）。两组间C（-106）T基因型无显著差异（P=0.40）。与野生型基因型相比，杂合子CT基因型患DR风险的比值比（OR）为0.7（95%CI,0.38-1.3），纯合子TT基因型为0.76（95%CI,0.18-3.25）。DR风险与微量白蛋白尿（OR=4.61；95%CI,2.34-9.05）和胰岛素治疗（OR=3.43；95%CI,1.94-6.09）呈正相关。