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蛋白酶体组装伴侣Nas2 PDZ结构域的1.15埃分辨率结构

1.15 Å resolution structure of the proteasome-assembly chaperone Nas2 PDZ domain.

作者信息

Singh Chingakham R, Lovell Scott, Mehzabeen Nurjahan, Chowdhury Wasimul Q, Geanes Eric S, Battaile Kevin P, Roelofs Jeroen

机构信息

Division of Biology, Kansas State University, 338 Ackert Hall, Manhattan, KS 66506, USA.

Protein Structure Laboratory, University of Kansas, Del Shankel Structural Biology Center, Lawrence, KS 66047, USA.

出版信息

Acta Crystallogr F Struct Biol Commun. 2014 Apr;70(Pt 4):418-23. doi: 10.1107/S2053230X14003884. Epub 2014 Mar 25.

DOI:10.1107/S2053230X14003884
PMID:24699731
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3976055/
Abstract

The 26S proteasome is a 2.5 MDa protease dedicated to the degradation of ubiquitinated proteins in eukaryotes. The assembly of this complex containing 66 polypeptides is assisted by at least nine proteasome-specific chaperones. One of these, Nas2, binds to the proteasomal AAA-ATPase subunit Rpt5. The PDZ domain of Nas2 binds to the C-terminal tail of Rpt5; however, it does not require the C-terminus of Rpt5 for binding. Here, the 1.15 Å resolution structure of the PDZ domain of Nas2 is reported. This structure will provide a basis for further insights regarding the structure and function of Nas2 in proteasome assembly.

摘要

26S蛋白酶体是一种2.5兆道尔顿的蛋白酶，专门负责真核生物中泛素化蛋白的降解。这种包含66种多肽的复合物的组装由至少九种蛋白酶体特异性伴侣蛋白协助。其中之一Nas2与蛋白酶体的AAA - ATP酶亚基Rpt5结合。Nas2的PDZ结构域与Rpt5的C末端尾巴结合；然而，它结合Rpt5并不需要Rpt5的C末端。在此，报道了Nas2的PDZ结构域分辨率为1.15埃的结构。该结构将为进一步深入了解Nas2在蛋白酶体组装中的结构和功能提供基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d97/3976055/a46ecee450df/f-70-00418-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d97/3976055/921014a92783/f-70-00418-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d97/3976055/b23fd7784e73/f-70-00418-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d97/3976055/a46ecee450df/f-70-00418-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d97/3976055/921014a92783/f-70-00418-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d97/3976055/b23fd7784e73/f-70-00418-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d97/3976055/a46ecee450df/f-70-00418-fig3.jpg

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本文引用的文献

1
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Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1204-14. doi: 10.1107/S0907444913000061. Epub 2013 Jun 13.
3
Reconfiguration of the proteasome during chaperone-mediated assembly.伴侣分子介导组装过程中蛋白酶体的重配置。
泛素依赖性开关在由 Not4 泛素连接酶介导的蛋白酶体 ATP 酶组装过程中。
Proc Natl Acad Sci U S A. 2018 Dec 26;115(52):13246-13251. doi: 10.1073/pnas.1805353115. Epub 2018 Dec 10.
Nature. 2013 May 23;497(7450):512-6. doi: 10.1038/nature12123. Epub 2013 May 5.
4
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5
Near-atomic resolution structural model of the yeast 26S proteasome.酵母 26S 蛋白酶体的近原子分辨率结构模型。
Proc Natl Acad Sci U S A. 2012 Sep 11;109(37):14870-5. doi: 10.1073/pnas.1213333109. Epub 2012 Aug 27.
6
Linking crystallographic model and data quality.链接晶体学模型和数据质量。
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