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参与质粒R1复制调控的RepA mRNA前导转录本的二级结构分析。

Secondary structure analysis of the RepA mRNA leader transcript involved in control of replication of plasmid R1.

作者信息

Ohman M, Wagner E G

机构信息

Department of Microbiology, Uppsala University, Sweden.

出版信息

Nucleic Acids Res. 1989 Apr 11;17(7):2557-79. doi: 10.1093/nar/17.7.2557.

DOI:10.1093/nar/17.7.2557
PMID:2470028
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC317643/
Abstract

The main replication control function in plasmid R1 is an antisense RNA, CopA RNA. By binding to its target (CopT) in the leader of the RepA mRNA, CopA RNA inhibits the expression of the rate-limiting RepA protein. The formation of the RNA duplex has been proposed to alter the folding around the RepA start region. Knowledge of the secondary structure of both CopA and CopT RNA is crucial for an understanding of the regulation. Previously, we reported the structure of CopA RNA under native conditions. In the present communication we have analyzed the secondary structure of the RepA leader transcript. Our main findings are: The two loops of CopA RNA have their correspondence in CopT RNA. No major structural changes are found downstream of the duplex when CopA was bound to its target RNA during transcription. Furthermore, in agreement with CopA/CopT RNA binding studies reported recently we do not find evidence for the existence of a binding window.

摘要

质粒R1中的主要复制控制功能由一种反义RNA，即CopA RNA发挥。通过与RepA mRNA前导区的靶标（CopT）结合，CopA RNA抑制限速性RepA蛋白的表达。有人提出RNA双链体的形成会改变RepA起始区域周围的折叠。了解CopA和CopT RNA的二级结构对于理解这种调控至关重要。此前，我们报道了天然条件下CopA RNA的结构。在本通讯中，我们分析了RepA前导转录本的二级结构。我们的主要发现如下：CopA RNA的两个环在CopT RNA中有对应结构。转录过程中当CopA与其靶标RNA结合时，在双链体下游未发现重大结构变化。此外，与最近报道的CopA/CopT RNA结合研究一致，我们没有发现存在结合窗口的证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/1b7eac756e2f/nar00124-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/98eca4a6d1a0/nar00124-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/608decb88cf3/nar00124-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/a1aaa7db5278/nar00124-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/1ae4b35f170d/nar00124-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/6a53766a348a/nar00124-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/d1f87d6774bf/nar00124-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/1b7eac756e2f/nar00124-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/98eca4a6d1a0/nar00124-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/608decb88cf3/nar00124-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/a1aaa7db5278/nar00124-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/1ae4b35f170d/nar00124-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/6a53766a348a/nar00124-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/d1f87d6774bf/nar00124-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c1e/317643/1b7eac756e2f/nar00124-0203-a.jpg

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本文引用的文献

1
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EMBO J. 1983;2(1):93-8. doi: 10.1002/j.1460-2075.1983.tb01387.x.
2
Posttranscriptional modification of mRNA conformation: mechanism that regulates erythromycin-induced resistance.mRNA构象的转录后修饰：调节红霉素诱导抗性的机制
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7079-83. doi: 10.1073/pnas.77.12.7079.
3
Translational control of IS10 transposition.IS10转座的翻译调控
Nucleic Acids Res. 2001 Jan 1;29(1):189-93. doi: 10.1093/nar/29.1.189.
4
Progression of a loop-loop complex to a four-way junction is crucial for the activity of a regulatory antisense RNA.环-环复合物向四链体连接的转变对于一种调控性反义RNA的活性至关重要。
EMBO J. 2000 Nov 1;19(21):5905-15. doi: 10.1093/emboj/19.21.5905.
5
An unusual structure formed by antisense-target RNA binding involves an extended kissing complex with a four-way junction and a side-by-side helical alignment.由反义靶RNA结合形成的一种异常结构涉及一个具有四向连接和并排螺旋排列的延伸亲吻复合体。
RNA. 2000 Mar;6(3):311-24. doi: 10.1017/s135583820099215x.
6
Suppression of replication-deficient mutants of IncFII plasmid NR1 can occur by two different mechanisms that increase expression of the repA1 gene.FII型质粒NR1复制缺陷型突变体的抑制可通过两种不同机制发生，这两种机制均可增加repA1基因的表达。
J Bacteriol. 1993 May;175(10):3161-73. doi: 10.1128/jb.175.10.3161-3173.1993.
7
Mechanism of post-segregational killing: translation of Hok, SrnB and Pnd mRNAs of plasmids R1, F and R483 is activated by 3'-end processing.后分离杀伤机制：质粒R1、F和R483的hok、SrnB和Pnd mRNA的翻译通过3'端加工被激活。
EMBO J. 1994 Apr 15;13(8):1950-9. doi: 10.1002/j.1460-2075.1994.tb06464.x.
8
Interaction between the antisense and target RNAs involved in the regulation of IncB plasmid replication.参与IncB质粒复制调控的反义RNA与靶RNA之间的相互作用。
J Bacteriol. 1993 May;175(10):2895-906. doi: 10.1128/jb.175.10.2895-2906.1993.
9
Activation of alpha-toxin translation in Staphylococcus aureus by the trans-encoded antisense RNA, RNAIII.反式编码的反义RNA（RNAIII）激活金黄色葡萄球菌中α毒素的翻译。
EMBO J. 1995 Sep 15;14(18):4569-77. doi: 10.1002/j.1460-2075.1995.tb00136.x.
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J Bacteriol. 1995 Aug;177(16):4730-41. doi: 10.1128/jb.177.16.4730-4741.1995.
Cell. 1983 Sep;34(2):683-91. doi: 10.1016/0092-8674(83)90401-4.
4
Analysis of plasmid genome evolution based on nucleotide-sequence comparison of two related plasmids of Escherichia coli.基于大肠杆菌两个相关质粒核苷酸序列比较的质粒基因组进化分析。
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5
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J Bacteriol. 1984 Oct;160(1):28-35. doi: 10.1128/jb.160.1.28-35.1984.
6
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Cell. 1984 Feb;36(2):513-22. doi: 10.1016/0092-8674(84)90244-7.
7
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Proc Natl Acad Sci U S A. 1981 Oct;78(10):6096-100. doi: 10.1073/pnas.78.10.6096.
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RNAs involved in copy-number control and incompatibility of plasmid R1.参与质粒R1拷贝数控制和不相容性的RNA
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10
Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.利用热力学和辅助信息对大型RNA序列进行最优计算机折叠
Nucleic Acids Res. 1981 Jan 10;9(1):133-48. doi: 10.1093/nar/9.1.133.