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参与IncB质粒复制调控的反义RNA与靶RNA之间的相互作用。

Interaction between the antisense and target RNAs involved in the regulation of IncB plasmid replication.

作者信息

Siemering K R, Praszkier J, Pittard A J

机构信息

Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.

出版信息

J Bacteriol. 1993 May;175(10):2895-906. doi: 10.1128/jb.175.10.2895-2906.1993.

DOI:10.1128/jb.175.10.2895-2906.1993
PMID:7684039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC204607/
Abstract

Physical analysis of RNA I, the small antisense RNA which regulates the replication of IncB miniplasmid pMU720, showed that it is a highly structured molecule containing an imperfectly paired stem closed by a 6-base hairpin loop. Mutational studies revealed that a 3-base sequence in the hairpin loop is critical to the interaction between RNA I and its complementary target in the RepA mRNA (RNA II). Furthermore, a 2-base interior loop in the upper stem was found to play an important role in facilitating effective binding between RNA I and RNA II. From these analyses, a model describing the molecular mechanism of binding between RNA I and RNA II is proposed.

摘要

对RNA I（一种调控IncB小质粒pMU720复制的小反义RNA）的物理分析表明，它是一种高度结构化的分子，含有一个由6个碱基的发夹环封闭的不完全配对茎。突变研究表明，发夹环中的一个3碱基序列对于RNA I与其在RepA mRNA（RNA II）中的互补靶标之间的相互作用至关重要。此外，发现上部茎中的一个2碱基内环在促进RNA I与RNA II之间的有效结合中起重要作用。基于这些分析，提出了一个描述RNA I与RNA II之间结合分子机制的模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/204607/7c72d43d4310/jbacter00052-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/204607/7c72d43d4310/jbacter00052-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/883e/204607/7c72d43d4310/jbacter00052-0116-a.jpg

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