Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071 (PR China).
Angew Chem Int Ed Engl. 2014 May 26;53(22):5624-8. doi: 10.1002/anie.201311203. Epub 2014 Apr 2.
Solid-state NMR is a powerful tool for studying membrane proteins in a native-like lipid environment. 3D magic angle spinning (MAS) NMR was employed to characterize the structure of E.coli diacylglycerol kinase (DAGK) reconstituted into its native E.coli lipid membranes. The secondary structure and topology of DAGK revealed by solid-state NMR are different from those determined by solution-state NMR and X-ray crystallography. This study provides a good example for demonstrating the influence of membrane environments on the structure of membrane proteins.
固态 NMR 是研究天然脂质环境中膜蛋白的有力工具。3D 魔角旋转（MAS）NMR 被用于表征重组到其天然大肠杆菌脂质膜中的大肠杆菌二酰基甘油激酶（DAGK）的结构。固态 NMR 揭示的 DAGK 的二级结构和拓扑结构与溶液态 NMR 和 X 射线晶体学测定的结果不同。这项研究为展示膜环境对膜蛋白结构的影响提供了一个很好的例子。
