1型糖尿病两名患者TCR Vβ7的计算机模拟分析
In silico Analysis of TCR Vβ7 of Two Patients with Type 1 Diabetes Mellitus.
作者信息
Zhou Jianwei, Kong Cui, Wang Xiukui, Jia Yinfeng, Wang Li, Chang Hong, Sun Lin
机构信息
Clinic Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China.
Department of Cardiovascular Disease, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China.
出版信息
J Lab Physicians. 2013 Jul;5(2):79-82. doi: 10.4103/0974-2727.119845.
OBJECTIVE
To compare the sequences and crystal structures of variable region of beta chain 7 (Vβ7) of T cell receptor (TCR) of two patients with type 1 diabetes mellitus (T1DM).
PATIENTS AND METHODS
The skewness of TCR Vβ7 of two T1DM patients were detected with real-time florescence quantitative polymerase chain reaction (FQ-PCR) and deoxyribonucleic acid (DNA) melting curve analysis technique followed by being sequenced, the crystal structures of them were simulated according to CPH models 2.0 Server, IMGT database, and RasMol 2 software.
RESULTS
The whole sequences of TCR Vβ7 of T1DM patient-1 were "CASRTAGQYEQYFGPGTR", that of patient-2 were "CASRTAGQYEQFFGPGTR"; the only difference between them lied on the 12(th) amino acid. The crystal structures of Vβ7 of the two patients simulated with backbone model were rather similar, while that with sphere model were obviously different.
CONCLUSION
Although the TCR Vβ7 of the T1DM patients share the similar gene sequences, their crystal structures simulated with sphere model are different, and the mechanism needs further study.
目的
比较两名1型糖尿病（T1DM）患者T细胞受体（TCR）β链7可变区（Vβ7）的序列和晶体结构。
患者与方法
采用实时荧光定量聚合酶链反应（FQ-PCR）和脱氧核糖核酸（DNA）熔解曲线分析技术检测两名T1DM患者TCR Vβ7的偏斜度，随后进行测序，并根据CPH模型2.0服务器、国际免疫基因组信息系统（IMGT）数据库和RasMol 2软件模拟其晶体结构。
结果
T1DM患者1的TCR Vβ7全序列为“CASRTAGQYEQYFGPGTR”，患者2的为“CASRTAGQYEQFFGPGTR”；两者唯一的差异在于第12位氨基酸。用主链模型模拟的两名患者Vβ7的晶体结构相当相似，而用球体模型模拟的则明显不同。
结论
虽然T1DM患者的TCR Vβ7具有相似的基因序列，但用球体模型模拟的其晶体结构不同，其机制有待进一步研究。
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