比较结核患儿和卡介苗接种儿童 T 细胞受体 β 链可变基因谱偏度。

Comparing TCR beta chain variable gene profile skewness between children with tuberculosis and BCG-vaccinated children.

机构信息

Clinic Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China.

出版信息

Arch Iran Med. 2013 Feb;16(2):104-8.

Abstract

BACKGROUND

We compared the T cell antigen receptor (TCR-BV) gene families of peripheral blood mononuclear cells (PMBC) between children with tuberculosis (TB) and those inoculated with the Bacille Calmette Guerin (BCG) vaccine.

METHODS

The total RNA was extracted from PMBC of 15 TB children, 15 BCG-vaccinated children and 15 healthy controls. The RNAs were reverse-transcribed and amplified by polymerase chain reaction (PCR). PCR products were separated on 1.5% agarose gel and analyzed with the Genescan technique.

RESULTS

Some TCR-BV gene families in TB children and BCG-vaccinated children exhibited a blur band in the predicted position on 1.5% agarose gel, some showed a distinct or fainted band. In general, many shared predominant clonal TCR-BV gene families (Vβ2, Vβ16, Vβ21, Vβ22) and the restricted-expression families (Vβ14 and Vβ17). All the gene families of the control children only exhibited blur bands and polyclonal.

CONCLUSIONS

The skewed profile of TCR-BV gene families in TB children and BCG-vaccinated children are similar, which may probably explain the protective effects of BCG-vaccine against TB in children.

摘要

背景

我们比较了肺结核（TB）患儿和卡介苗（BCG）接种儿童外周血单个核细胞（PMBC）中的 T 细胞抗原受体（TCR-BV）基因家族。

方法

从 15 例 TB 患儿、15 例 BCG 接种儿童和 15 例健康对照的 PMBC 中提取总 RNA。用聚合酶链反应（PCR）将 RNA 逆转录并扩增。将 PCR 产物在 1.5%琼脂糖凝胶上分离，并使用 Genescan 技术进行分析。

结果

TB 患儿和 BCG 接种儿童的一些 TCR-BV 基因家族在 1.5%琼脂糖凝胶的预测位置显示模糊带，一些则显示明显或模糊带。一般来说，许多共享主要的克隆 TCR-BV 基因家族（Vβ2、Vβ16、Vβ21、Vβ22）和限制性表达家族（Vβ14 和 Vβ17）。所有对照儿童的基因家族仅显示模糊带和多克隆。