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Stereochemical course of catalysis by the Tetrahymena ribozyme.

作者信息

Rajagopal J, Doudna J A, Szostak J W

机构信息

Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.

出版信息

Science. 1989 May 12;244(4905):692-4. doi: 10.1126/science.2470151.

DOI:10.1126/science.2470151
PMID:2470151
Abstract

The group I intron from Tetrahymena catalyzes phosphodiester transfer reactions on various RNA substrates. A modified RNA substrate with a phosphorothioate group in one stereoisomeric form at the site of reaction was synthesized in order to determine the stereochemical course of an RNA-catalyzed reaction. The reaction product was digested with a stereospecific nuclease to determine the configuration of the product phosphorothioate. The reaction occurs with inversion of configuration at phosphorus, implying an in-line pathway for the reaction.

摘要

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