Been M D, Cech T R
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.
Science. 1988 Mar 18;239(4846):1412-6. doi: 10.1126/science.2450400.
A catalytic RNA (ribozyme) derived from an intervening sequence (IVS) RNA of Tetrahymena thermophila will catalyze an RNA polymerization reaction in which pentacytidylic acid (C5) is extended by the successive addition of mononucleotides derived from a guanylyl-(3',5')-nucleotide (GpN). Cytidines or uridines are added to C5 to generate chain lengths of 10 to 11 nucleotides, with longer products being generated at greatly reduced efficiency. The reaction is analogous to that catalyzed by a replicase with C5 acting as the primer, GpNs as the nucleoside triphosphates, and a sequence in the ribozyme providing a template. The demonstration that an RNA enzyme can catalyze net elongation of an RNA primer supports theories of prebiotic RNA self-replication.
一种源自嗜热四膜虫居间序列（IVS）RNA的催化性RNA（核酶），将催化一种RNA聚合反应，其中五聚胞苷酸（C5）通过依次添加源自鸟苷酰-（3',5'）-核苷酸（GpN）的单核苷酸而得到延伸。胞苷或尿苷被添加到C5上，以产生10至11个核苷酸的链长，更长的产物生成效率则大幅降低。该反应类似于由复制酶催化的反应，其中C5作为引物，GpN作为核苷三磷酸，核酶中的一个序列提供模板。RNA酶能够催化RNA引物净延伸这一证明，支持了前生物RNA自我复制的理论。
