Li Qian, Zhao Xinqing, Kim Jin-Soo, Bai Fengwu
Sheng Wu Gong Cheng Xue Bao. 2013 Nov;29(11):1672-5.
Ethanol tolerance is related to the expression of multiple genes, and genome-based engineering approaches are much more efficient than manipulation of single genes. In this study, ultraviolet (UV) mutagenesis, dielectric barrier discharge (DBD) air plasma mutagenesis, and artificial transcription factor (ATF) technology were adopted to treat an industrial yeast strain S. cerevisiae Sc4126 to obtain mutants with improved ethanol tolerance. Mutants with high ethanol tolerance were obtained, and the ratio of positive mutants was compared. Among the three approaches, the rate of positive mutation obtained by ATF technology was 10- to 100-folds of that of the two other methods, with highest genetic stability, suggesting the ATF technology promising for rapid alteration of phenotypes of industry yeast strains for efficient ethanol fermentation.
乙醇耐受性与多个基因的表达相关，基于基因组的工程方法比单个基因的操作效率更高。在本研究中，采用紫外线（UV）诱变、介质阻挡放电（DBD）空气等离子体诱变和人工转录因子（ATF）技术处理工业酵母菌株酿酒酵母Sc4126，以获得乙醇耐受性提高的突变体。获得了高乙醇耐受性的突变体，并比较了阳性突变体的比例。在这三种方法中，ATF技术获得的阳性突变率是其他两种方法的10到100倍，遗传稳定性最高，这表明ATF技术有望快速改变工业酵母菌株的表型，实现高效乙醇发酵。
