人神经祖细胞源性螺旋神经节样神经元的分化：一项延时视频研究。
Differentiation of human neural progenitor cell-derived spiral ganglion-like neurons: a time-lapse video study.
作者信息
Edin Fredrik, Liu Wei, Boström Marja, Magnusson Peetra U, Rask-Andersen Helge
机构信息
Department of Surgical Sciences, Section for Otolaryngology and Head- and Neck surgery, Uppsala University and Uppsala University Hospital.
出版信息
Acta Otolaryngol. 2014 May;134(5):441-7. doi: 10.3109/00016489.2013.875220.
CONCLUSIONS
Human neural progenitor cells can differentiate into spiral ganglion-like cells when exposed to inner ear-associated growth factors. The phenotype bears resemblance to human sphere-derived neurons.
OBJECTIVE
To establish an in vitro model for the human auditory nerve to replace and complement in vivo animal experiments and ultimately human in vivo transplantation.
METHODS
Human neural progenitors were differentiated under conditions developed for in vitro survival of human primary spiral ganglion culture with media containing growth factors associated with inner ear development. Differentiation was documented using time-lapse video microscopy. Time-dependent marker expression was evaluated using immunocytochemistry with fluorescence and laser confocal microscopy.
RESULTS
Within 14 days of differentiation, neural progenitors adopted neural phenotype and expressed spiral ganglion-associated markers.
结论
当暴露于内耳相关生长因子时，人神经祖细胞可分化为螺旋神经节样细胞。该表型与源自人神经球的神经元相似。
目的
建立人听神经的体外模型，以替代和补充体内动物实验，并最终用于人体体内移植。
方法
在为人类原代螺旋神经节培养的体外存活而开发的条件下，使用含有与内耳发育相关生长因子的培养基使人神经祖细胞分化。使用延时视频显微镜记录分化过程。使用荧光免疫细胞化学和激光共聚焦显微镜评估时间依赖性标志物表达。
结果
在分化的14天内，神经祖细胞呈现神经表型并表达螺旋神经节相关标志物。
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