Baenkler H W, Lux G
Department of Medicine, University Hospital, Erlangen, Germany.
Ann Allergy. 1989 May;62(5):449-52.
Twenty-four patients allergic to food, which was demonstrated by oral provocation, were investigated. Six particles from duodenal mucosa were obtained during endoscopic examination and incubated with different foods. Specimens challenged by anti-human-IgE or without any stimulus served as control values. Also, skin tests and assays of specific IgE were performed. The spontaneous histamine release varied from 19% to 36%. Anti-IgE caused an increase up to 26% until 65%. Incubation with allergenic food induced a histamine release from 41% to 81%, demonstrating positive results in 27 out of 30 separate experiments. Antigen-induced histamine release from biopsy specimens turned out superior to skin tests and specific IgE. It is the most reliable tool for diagnosis of gastrointestinal food allergy besides oral provocation.
对24名经口服激发试验证实对食物过敏的患者进行了研究。在内镜检查期间从十二指肠黏膜获取6个颗粒，并与不同食物一起孵育。用抗人IgE激发的标本或未受任何刺激的标本作为对照值。此外，还进行了皮肤试验和特异性IgE检测。组胺自发释放率在19%至36%之间。抗IgE可使组胺释放增加至26%，最高可达65%。与致敏食物孵育可诱导组胺释放率从41%至81%，在30次单独实验中有27次显示阳性结果。活检标本中抗原诱导的组胺释放结果优于皮肤试验和特异性IgE。除口服激发试验外，它是诊断胃肠道食物过敏最可靠的工具。
