腺病毒介导的α-烯醇化酶基因产物上调对鹅F1卵泡颗粒细胞促卵泡激素受体mRNA和促黄体生成素受体mRNA的影响。

Effect of adenovirus-mediated up-regulation of α-enolase gene products on follicle-stimulating hormone receptor mRNA and luteinizing hormone receptor mRNA of granular cells from goose F1 follicles.

作者信息

Ji Hong, Yang Huanmin, Wang Jianfa, Guo Jingru, Hu Zhongming, Zhang Hongliang, Kong Fanzhi, Zhen Li, Guo Li, Liu Juxiong

机构信息

College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin Province, China; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.

出版信息

Res Vet Sci. 2014 Jun;96(3):526-32. doi: 10.1016/j.rvsc.2014.02.010. Epub 2014 Feb 27.

DOI:10.1016/j.rvsc.2014.02.010

PMID:24703612

Abstract

Enolases are glycolytic enzymes in the glycolytic pathway. In order to evaluate the effect of ENO1 on follicle-stimulating hormone receptor (FSHR) mRNA and luteinizing hormone receptor (LHR) mRNA of primary granular cell from goose F1 follicles, the recombinant plasmid adenovirus carrying ENO1 were constructed and infected the primary culture granular cells. The granular cells were randomly divided into three groups: recombinant adenovirus infected (pAd-CMV-ENO1), empty vector infected (pAd-CMV-Null) and no virus (mock control). The expression levels of FSHR mRNA and LHR mRNA of granular cells were examined by qRT-PCR. The results showed the group pAd-CMV-ENO1 had significantly higher FSHR mRNA expression levels than the other two groups (P < 0.05), but had significantly lower LHR mRNA expression levels than the other two groups (P < 0.05). The results suggested that ENO1 could improve the combination rate between FSH and FSHR to accelerate the proliferation and differentiation and steroidogenesis in poultry gonadal tissues.

摘要

烯醇化酶是糖酵解途径中的糖酵解酶。为了评估ENO1对鹅F1卵泡原代颗粒细胞中促卵泡激素受体（FSHR）mRNA和促黄体生成素受体（LHR）mRNA的影响，构建了携带ENO1的重组质粒腺病毒并感染原代培养的颗粒细胞。颗粒细胞被随机分为三组：重组腺病毒感染组（pAd-CMV-ENO1）、空载体感染组（pAd-CMV-Null）和无病毒组（模拟对照）。通过qRT-PCR检测颗粒细胞中FSHR mRNA和LHR mRNA的表达水平。结果显示，pAd-CMV-ENO1组的FSHR mRNA表达水平显著高于其他两组（P<0.05），但LHR mRNA表达水平显著低于其他两组（P<0.05）。结果表明，ENO1可以提高FSH与FSHR之间的结合率，以加速家禽性腺组织的增殖、分化和类固醇生成。