Nguyen Loan To, Lau Li Yieng, Fortes Marina Rufino Salinas
Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Australia.
Agency of Science, Technology and Research, Singapore, Singapore.
Front Genet. 2022 Jun 14;13:935433. doi: 10.3389/fgene.2022.935433. eCollection 2022.
The hypothalamus and the pituitary gland are directly involved in the complex systemic changes that drive the onset of puberty in cattle. Here, we applied integrated bioinformatics to elucidate the critical proteins underlying puberty and uncover potential molecular mechanisms from the hypothalamus and pituitary gland of prepubertal ( = 6) and postpubertal ( = 6) cattle. Proteomic analysis in the hypothalamus and pituitary gland revealed 275 and 186 differentially abundant (DA) proteins, respectively (adjusted -value < 0.01). The proteome profiles found herein were integrated with previously acquired transcriptome profiles. These transcriptomic studies used the same tissues harvested from the same heifers at pre- and post-puberty. This comparison detected a small number of matched transcripts and protein changes at puberty in each tissue, suggesting the need for multiple omics analyses for interpreting complex biological systems. In the hypothalamus, upregulated DA proteins at post-puberty were enriched in pathways related to puberty, including , whereas downregulated proteins were observed in the . Additionally, this study revealed that ribosomal pathway proteins in the pituitary were involved in the pubertal development of mammals. The reported molecules and derived protein-protein networks are a starting point for future experimental approaches that might dissect with more detail the role of each molecule to provide new insights into the mechanisms of puberty onset in cattle.
下丘脑和垂体直接参与了驱动牛青春期开始的复杂全身变化。在此，我们应用综合生物信息学来阐明青春期潜在的关键蛋白质，并从青春期前（n = 6）和青春期后（n = 6）牛的下丘脑和垂体中揭示潜在的分子机制。下丘脑和垂体的蛋白质组学分析分别揭示了275种和186种差异丰富（DA）蛋白质（校正P值<0.01）。本文发现的蛋白质组图谱与先前获得的转录组图谱进行了整合。这些转录组学研究使用了从同一小母牛青春期前和青春期后采集的相同组织。这种比较在每个组织中检测到青春期时有少量匹配的转录本和蛋白质变化，这表明需要进行多种组学分析来解释复杂的生物系统。在下丘脑中，青春期后上调的DA蛋白质在与青春期相关的途径中富集，包括……，而在……中观察到下调的蛋白质。此外，本研究表明垂体中的核糖体途径蛋白质参与了哺乳动物的青春期发育。报道的分子和衍生的蛋白质-蛋白质网络是未来实验方法的起点，这些方法可能会更详细地剖析每个分子的作用，从而为牛青春期开始的机制提供新的见解。
