Kantani-Matsumoto A, Kataoka K
Department of Anatomy, Hiroshima University School of Medicine, Japan.
Arch Histol Cytol. 1989 Mar;52(1):37-50. doi: 10.1679/aohc.52.37.
The ontogenesis of the mouse gastric mucosa was studied by carbohydrate histochemistry and 3H-thymidine autoradiography. Surface mucous cells and glandular cells were identified from day 16 of gestation. Sugar residues in the mucin of surface mucous cells seem to undergo no major changes throughout the period under study, since secretory granules of the cells were positive in periodic acid-Schiff (PAS) and galactose oxidase-Schiff (GOS) reactions and consistently bound certain lectins. Chief cells and mucous neck cells are not separated until the third postnatal week, though primitive chief cells are present during earlier developmental stages. Secretory granules of primitive chief cells shared positive PAS and GOS reactions with mucous neck cells and bound similar lectins, but the intensity was generally weaker. The granules of primitive chief cells were also stained by Bowie's solution which exclusively stained zymogen granules in chief cells in adults. These results suggest that secretory granules of primitive chief cells contain a complex carbohydrate similar to mucin in mucous neck cells, but with a lower sugar/protein ratio. It is concluded by studies using 3H-thymidine autoradiography combined with carbohydrate histochemistry that, though immature surface mucous cells, primitive chief cells and mucous neck cells actively proliferate, chief cells rarely undergo mitosis.
通过碳水化合物组织化学和3H-胸腺嘧啶核苷放射自显影术研究了小鼠胃黏膜的个体发生。从妊娠第16天开始识别表面黏液细胞和腺细胞。在整个研究期间，表面黏液细胞黏蛋白中的糖残基似乎没有发生重大变化，因为这些细胞的分泌颗粒在过碘酸希夫（PAS）反应和半乳糖氧化酶希夫（GOS）反应中呈阳性，并始终结合某些凝集素。主细胞和黏液颈细胞直到出生后第三周才分开，尽管在早期发育阶段就存在原始主细胞。原始主细胞的分泌颗粒与黏液颈细胞在PAS和GOS反应中呈阳性，并结合相似的凝集素，但强度通常较弱。原始主细胞的颗粒也被鲍伊氏溶液染色，该溶液专门染成人大主细胞中的酶原颗粒。这些结果表明，原始主细胞的分泌颗粒含有一种类似于黏液颈细胞黏蛋白的复合碳水化合物，但糖/蛋白比例较低。通过结合碳水化合物组织化学的3H-胸腺嘧啶核苷放射自显影术研究得出结论，尽管未成熟的表面黏液细胞、原始主细胞和黏液颈细胞活跃增殖，但主细胞很少进行有丝分裂。
