Suzuki S, Tsuyama S, Murata F
Cell Tissue Res. 1983;233(3):475-84. doi: 10.1007/BF00212218.
Morphological and histochemical methods revealed cells intermediate between mucous neck cells and chief cells in rat stomach. The secretory granules of mucous neck cells and chief cells were homogeneous. However, some cells resembling mucous neck cells in shape and size yet contained heterogeneous granules. One type of cell contained mucous granules showing a bipartite structure. The inner cores of these granules resembled the granules of mucous neck cells morphologically and after staining by the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) procedure and lectin-ferritin methods. The outer layer of these granules, however, resembled the granules of chief cells and failed to stain by these methods. Another type of cell also contained bipartite granules, but these showed a dense cap and lucent matrix. The cap of the granules did not stain with the PA-TCH-SP and lectin methods, but the remaining matrix was reactive. These presumed transitional cells were located in a layer between the mucous neck cells and the chief cells. Immature chief cells with sparse granular reticulum were found near these intermediate cells in some cases. Many similar intermediate type cells were also found in early developmental stages of the gastric mucosa in rat. These results suggest a close relationship between chief cells and mucous neck cells; chief cells may develop from undifferentiated stem cells via mucous neck cells in adult rat stomach.
形态学和组织化学方法揭示了大鼠胃中介于黏液颈细胞和主细胞之间的细胞。黏液颈细胞和主细胞的分泌颗粒是均匀一致的。然而，一些在形状和大小上类似于黏液颈细胞的细胞却含有异质性颗粒。一种类型的细胞含有呈现二分结构的黏液颗粒。这些颗粒的内核在形态上以及经高碘酸-硫代碳酰肼-银蛋白（PA-TCH-SP）法和凝集素-铁蛋白法染色后类似于黏液颈细胞的颗粒。然而，这些颗粒的外层类似于主细胞的颗粒，并且用这些方法无法染色。另一种类型的细胞也含有二分颗粒，但这些颗粒显示出致密的帽和透明的基质。颗粒的帽用PA-TCH-SP法和凝集素法不染色，但其余的基质有反应性。这些推测的过渡细胞位于黏液颈细胞和主细胞之间的一层中。在某些情况下，在这些中间细胞附近发现了具有稀疏颗粒网状结构的未成熟主细胞。在大鼠胃黏膜的早期发育阶段也发现了许多类似的中间型细胞。这些结果表明主细胞和黏液颈细胞之间关系密切；在成年大鼠胃中，主细胞可能从未分化的干细胞经黏液颈细胞发育而来。
