Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, South Bend, IN, USA.
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, South Bend, IN, USA; Department of Chemistry and Biochemistry, Notre Dame University, Notre Dame, IN, USA; Eck Institute for Global Health, Notre Dame University, Notre Dame, IN, USA; Indiana University Melvin and Bren Simon Cancer Center, Indianapolis, IN, USA.
Gene. 2014 Jun 10;543(1):174-80. doi: 10.1016/j.gene.2014.04.007. Epub 2014 Apr 3.
ARID3B is a DNA binding protein that is overexpressed in neuroblastoma and ovarian cancer. To understand the extent that ARID3B participates in tumor development, we assessed protein expression of ARID3B in normal adult and malignant tissues. We found that ARID3B is highly expressed in differentiated layers of squamous epithelium. We also examined expression of an alternative splice form of ARID3B and found that it has similar but not identical expression patterns to the full length ARID3B isoform. ARID3B has two closely related paralogues, ARID3A and ARID3C. Each of these 3 family members exhibits different patterns of expression. Of the ARID3 family members, ARID3B is the most widely expressed and is particularly expressed in epithelium. In addition to examining normal tissue, we investigated ARID3B expression in a variety of tumor types. Most notably we found that ARID3B expression is decreased in esophagus and stomach tumors compared to normal corresponding tissues. Our results indicate that the different patterns of ARID3B in normal tissues translate into different roles for ARID3B in carcinomas.
ARID3B 是一种 DNA 结合蛋白，在神经母细胞瘤和卵巢癌中过表达。为了了解 ARID3B 在肿瘤发生发展中所起的作用，我们评估了 ARID3B 在正常成人和恶性组织中的蛋白表达情况。我们发现 ARID3B 在鳞状上皮的分化层中高度表达。我们还研究了 ARID3B 的一种选择性剪接形式的表达，发现它与全长 ARID3B 异构体具有相似但不完全相同的表达模式。ARID3B 有两个密切相关的同源物，ARID3A 和 ARID3C。这 3 个家族成员的每个成员都表现出不同的表达模式。在 ARID3 家族成员中，ARID3B 的表达最为广泛，特别是在上皮组织中表达。除了检查正常组织外，我们还研究了 ARID3B 在各种肿瘤类型中的表达情况。最值得注意的是，我们发现与正常相应组织相比，ARID3B 在食管和胃肿瘤中的表达降低。我们的结果表明，ARID3B 在正常组织中的不同表达模式转化为 ARID3B 在癌中的不同作用。
