Renaux Estelle, Baudouin Charlotte, Schakman Olivier, Gay Ondine, Martin Manon, Marchese Damien, Achouri Younès, Rezsohazy René, Gofflot Françoise, Clotman Frédéric
Université catholique de Louvain, Louvain Institute of Biomolecular Science and Technology, Animal Molecular and Cellular Biology group, Louvain-la-Neuve, Belgium.
Université catholique de Louvain, Institute of Neuroscience, Laboratory of Neural Differentiation, Brussels, Belgium.
Front Cell Neurosci. 2024 Oct 16;18:1466056. doi: 10.3389/fncel.2024.1466056. eCollection 2024.
Motor activity is organized by neuronal networks composed of motor neurons and a wide variety of pre-motor interneuron populations located in the brainstem and spinal cord. Differential expression and single-cell RNA sequencing studies recently unveiled that these populations subdivide into multiple subsets. However, some interneuron subsets have not been described yet, and the mechanisms contributing to this neuronal diversification have only been partly deciphered. In this study, we aimed to identify additional markers to further describe the diversity of spinal V2 interneuron populations. Here, we compared the transcriptome of V2 interneurons with that of the other cells of the embryonic spinal cord and extracted a list of genes enriched in V2 interneurons, including . Arid3c identifies an uncharacterized subset of V2 that partially overlaps with V2c interneurons. These two populations are characterized by the production of Onecut factors and Sox2, suggesting that they could represent a single functional V2 unit. Furthermore, we show that the overexpression or inactivation of does not alter V2 production, but its absence results in minor defects in locomotor execution, suggesting a possible function in subtle aspects of spinal locomotor circuit formation.
运动活动由神经网络组织，该网络由运动神经元以及位于脑干和脊髓中的多种运动前中间神经元群体组成。差异表达和单细胞RNA测序研究最近揭示，这些群体可细分为多个亚群。然而，一些中间神经元亚群尚未被描述，导致这种神经元多样化的机制也仅得到部分解读。在本研究中，我们旨在识别额外的标志物，以进一步描述脊髓V2中间神经元群体的多样性。在此，我们将V2中间神经元的转录组与胚胎脊髓的其他细胞的转录组进行了比较，并提取了在V2中间神经元中富集的基因列表，包括。Arid3c识别出V2的一个未被表征的亚群，该亚群与V2c中间神经元部分重叠。这两个群体的特征是产生Onecut因子和Sox2，表明它们可能代表一个单一的功能性V2单元。此外，我们表明，[此处原文缺失具体基因名称]的过表达或失活不会改变V2的产生，但其缺失会导致运动执行出现轻微缺陷,这表明其在脊髓运动回路形成的细微方面可能具有功能。
