Wei Shu-Jun, Li Qian, van Achterberg Kees, Chen Xue-Xin
State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China; Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
Mol Phylogenet Evol. 2014 Aug;77:1-10. doi: 10.1016/j.ympev.2014.03.023. Epub 2014 Apr 1.
In animal mitochondrial genomes, gene arrangements are usually conserved across major lineages but might be rearranged within derived groups, and might provide valuable phylogenetic characters. Here, we sequenced the mitochondrial genomes of Cephalonomia gallicola (Chrysidoidea: Bethylidae) and Wallacidia oculata (Vespoidea: Mutillidae). In Cephalonomia at least 11 tRNA and 2 protein-coding genes were rearranged, which is the first report of protein-coding gene rearrangements in the Aculeata. In the Hymenoptera, three types of protein-coding gene rearrangement events occur, i.e. reversal, transposition and reverse transposition. Venturia (Ichneumonidae) had the greatest number of common intervals with the ancestral gene arrangement pattern, whereas Philotrypesis (Agaonidae) had the fewest. The most similar rearrangement patterns are shared between Nasonia (Pteromalidae) and Philotrypesis, whereas the most differentiated rearrangements occur between Cotesia (Braconidae) and Philotrypesis. It is clear that protein-coding gene rearrangements in the Hymenoptera are evolutionarily independent across the major lineages but are conserved within groups such as the Chalcidoidea. Phylogenetic analyses supported the sister-group relationship of Orrussoidea and Apocrita, Ichneumonoidea and Aculeata, Vespidae and Apoidea, and the paraphyly of Vespoidea. The Evaniomorpha and phylogenetic relationships within Aculeata remain controversial, with discrepancy between analyses using protein-coding and RNA genes.
在动物线粒体基因组中，基因排列通常在主要谱系中保守，但在衍生类群中可能会发生重排，并且可能提供有价值的系统发育特征。在此，我们对栗头蜂虱（膜翅目：肿腿蜂科）和眼斑瓦氏蜂（膜翅目：蚁蜂科）的线粒体基因组进行了测序。在栗头蜂虱中，至少11个tRNA和2个蛋白质编码基因发生了重排，这是针尾部蛋白质编码基因重排的首次报道。在膜翅目中，发生了三种类型的蛋白质编码基因重排事件，即倒位、转座和反向转座。小腹茧蜂属（茧蜂科）与祖先基因排列模式的共同间隔数量最多，而榕小蜂属（榕小蜂科）的共同间隔数量最少。最相似的重排模式存在于丽蝇蛹集金小蜂（金小蜂科）和榕小蜂属之间，而差异最大的重排发生在茧蜂属（茧蜂科）和榕小蜂属之间。显然，膜翅目中蛋白质编码基因的重排在主要谱系中是独立进化的，但在小蜂总科等类群中是保守的。系统发育分析支持了细腰亚目与有翅亚纲、姬蜂总科与针尾部、胡蜂科与蜜蜂总科的姊妹群关系，以及胡蜂总科的并系性。细腰亚目的系统发育关系以及针尾部内部的系统发育关系仍存在争议，使用蛋白质编码基因和RNA基因进行的分析结果存在差异。
