Özçelik Hayriye, Padeste Celestino, Hasirci Vasif
METU, BIOMAT, Department of Biological Sciences, Biotechnology Research Unit, Ankara 06800, Turkey; BIOMATEN, METU Center of Excellence in Biomaterials and Tissue Engineering, Ankara 06800, Turkey.
Lab for Micro- and Nanotechnology, Paul Scherrer Institute, 5232 Villigen PSI, Switzerland.
Colloids Surf B Biointerfaces. 2014 Jul 1;119:71-81. doi: 10.1016/j.colsurfb.2014.03.019. Epub 2014 Mar 18.
Polymeric test surfaces of P(L-D,L)LA and of a P(L-D,L)LA:PLGA blend decorated with 25 nanopillar covered fields, were used to investigate differences in growth of bone marrow stem cells (BMSC) and osteosarcoma cells (Saos-2). The fields were populated with pillars (ca. 900 nm tall, 200 nm×200 nm area) separated systematically from each other with 1-10 μm gaps. Saos-2 cells populated fields decorated with pillars 1 μm apart but they avoided pillar-free surfaces. In contrast, BMSCs avoided fields with interpillar distances <2 μm. Both BMSCs and Saos-2 cells aligned in the direction of the shorter distance when at least one of the interpillar distances was greater than 1.5 μm. Coating the P(L-D,L)LA surfaces with cell adhesive protein fibronectin enabled the BMSC to populate fields with high pillar density which they had avoided when uncoated. Decreasing the stiffness of the film surface by using a blend of (P(L-D,L)LA and PLGA) made them more acceptable for attachment by the BMSC cells.
聚（L-D，L）乳酸以及含有25个纳米柱覆盖区域的聚（L-D，L）乳酸与聚乳酸-羟基乙酸共聚物（PLGA）共混物的聚合物测试表面，用于研究骨髓干细胞（BMSC）和成骨肉瘤细胞（Saos-2）生长的差异。这些区域布满了柱子（约900纳米高，200纳米×200纳米面积），柱子彼此之间系统地间隔着1-10微米的间隙。Saos-2细胞在间隔为1微米的柱子装饰的区域生长，但它们避开无柱子的表面。相比之下，BMSC避开柱子间距小于2微米的区域。当至少一个柱子间距大于1.5微米时，BMSC和Saos-2细胞都沿较短距离的方向排列。用细胞粘附蛋白纤连蛋白包被聚（L-D，L）乳酸表面，能使BMSC在高柱子密度的区域生长，而在未包被时它们会避开这些区域。通过使用聚（L-D，L）乳酸和PLGA的共混物降低薄膜表面的硬度，使其更易于被BMSC细胞附着。
